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A rapid, simple, and specific thin-layer chromatographic (TLC) method is described for estimation of various corticosteroids and their esters when present in combination with other drugs in pharmaceutical preparation of creams and ointments. The cream or ointment is dissolved in chloroform; water and insoluble material, if any, are removed by centrifugation, and the steroid is separated from other ingredients by TLC on silica gel G with n-hexane as the first developing solvent (to wash out cream or ointment base) and chloroform–ethyl acetate (1 + 1 , v/v, for free steroids or 2 + 1 , v/v, for steroid esters) as the second developing solvent. The separated steroid or ester is visualized under shortwave UV (254 nm) and estimated by spectrodensitometry at 240 nm. Recoveries of the steroids are between 98.0 and 101.7%. Results were compared with those obtained by official methods, where applicable, and excellent agreement was found.

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A densitometric and a spectrophotometric method for rapid but accurate determination of different components of bleomycin injections has been described. The bleomycin components were separated by reversed phase thin layer chromatography on silanized silicagel plates using a mixture of aqueous ammonium nitrate ( 2 . 5 % ) : methanol :: 7 : 3 (v/v) as mobile phase. Assay was done at the absorption maxima of the components (291 nm) by in situ densitometry or by spectroscopy after extracting the drugs from the adsorbent with the mobile phase. Results obtained by both the methods agreed well with each other and with those obtained by an official HPLC method. The densitometric method described was highly suitable for routine quality control of bleomycines as a large number of samples could be analysed within a short time ( 6 8 sanples/analyst/day).