Background: The Neckar River (Southern Germany) represents an aquatic system strongly affected by numerous anthropogenic activities. Thus, it is an excellent model for ecotoxicological investigations. The present study aims to assess time and spatial variations of embryo-and proteotoxic effects in surface water and sediment. For this end, embryos of zebrafish (Danio rerio) were exposed to Neckar River samples collected in the Tübingen region in different seasons over 2 years. Additionally, quantification of the heat shock (stress) protein Hsp70 was carried out in newly hatched larvae; furthermore, physico-chemical water parameters were measured in order to gain baseline information about the limnologic conditions.

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