The use of bio pesticides and organic pesticides from secondary metabolic filtrate of APH microorganisms is one of the food and horticulture cultivation technology packages which are the basis for this research objective. The research objective was to explore, isolate and identify of Streptomyces sp. in the land of shallot plants (Abm) and the antibiosis against the pathogens Fusariumsp cause of Moler shallot plant in vitro. Observer of Quality and quantity characteristics of secondary metabolites as antibiosis, The results showed that Streptomyces sp from land of shallot plant (Abm) isolates producing little antibiosis less able to inhibit the development of the pathogens Fusariumsp cause of Moler shallot plant in vitro. The identification of Actinomycetes spp. based on the morphology characteristic showed that Actinomycetes isolates are closely related with Streptomyces sp.