This work was carried out in collaboration between all authors. Author PS designed the study, wrotethe protocol, wrote the first draft of the manuscript and managed the literature searches, author Kusriningrum performed the statical analysis. Authors Ni'matuzaroh and TS managed the analysis of the study. All authors read and approved the final manuscript.
Fusarium oxysporum is a soil-borne fungus that attacks all stages of tomato plant from seedlings to mature plants. The disease occurrence on the tomato nursery can lead economically loss due to its fast and massive damage. Application of effective biological control agents is considered as promising control measure of the disease. The aim of this study was to evaluate the potential competitiveness of biological agents mixture consisting of Streptomyces sp., Gliocladium sp. and T. harzianum against F. oxysporum f. sp. lycopersici in the rhizosphere of the tomato plant. The experiment was arranged in completely randomized design with five treatment types of agents that a single biological agents Streptomyces sp. (S), a mixture of Streptomyces sp. and Gliocladium sp. (SG), a mixture of Streptomyces sp. and T. harzianum (ST), a mixture of Streptomyces sp., Gliocladium sp., and T. harzianum (SGT) and control without biological agents. Each treatment was replicated four times and consisted of 6 plants for observation purposes destructively. Descriptive analysis conducted on the pattern of colonization of the roots competition showed a growth pattern colonization of biological agents, Streptomyces sp, Gliocladium sp, T. harzianum and F. oxysporum pathogen (SGTF) was the same growth pattern of SGF and STF, where four of the microbes grew on the same side of roots as fungal pathogens, in all parts of the root sprouts. Given biological agent Streptomyces sp, Gliocladium sp., dan Trichoderma harzianum decreased fusarium wilt diseases severity of tomato. Weight root of tomato plants was applied by biological agents was 9.2 g greater than plant without biological agents, which were 2.5 g. The observation on tomato indicated root colonization occurs, especially on the base of the stem-roots by a mixture of biological agents T. harzianum and Gliocladium sp.
Streptomyces sp. and Trichoderma sp. are soil microorganisms isolated from shallot fields that can act as biological agents and increase crop production. Nezara viridula, the southern green stink bug, is the leading pest of soybean during the generative period, which can cause damage up to 80%. This study aimed to determine the efficacy of a liquid biopesticide formula using a mixture of coconut water and potato extract containing Streptomyces sp. and Trichoderma sp. This study used a randomized block design. The first factor was the time of application and the second factor was the concentration level. There were 8 treatment combinations and 2 controls. Each treatment combination was repeated three times. Probit LC50 and LT50 were performed to determine the effectiveness of biopesticides. The calculation of probit analysis obtained results of 84,443 ppm or about 84% for LC50, while the LT50 analysis obtained results of 4.7 days.
Perubahan iklim karena pemanasan global dapat meningkatkan suhu tanah. Peningkatan suhu tanah dapat dipengaruhi oleh suhu udara yang dapat mempengaruhi kehidupan mikroorganime tanah. Streptomyces sp. dan Trichoderma sp. adalah mikroorganisme tanah yang berperan sebagai Agen Pengendali Hayati (APH) hama dan penyakit tanaman. Penelitian ini bertujuan untuk mengetahui kemampuan daya hambat Streptomyces sp. dan Trichoderma sp. pada suhu tinggi terhadap Fusarium sp. penyebab penyakit layu pada cabai. Penelitian menggunakan rancangan acak lengkap faktorial. Faktor pertama adalah suhu ruang 29-31 °C, suhu 50 °C, dan suhu 70 °C. Faktor kedua adalah jenis tanah andosol (lahan UPN) dan regusol (lahan desa Pare). Pengamatan dilakukan terhadap jumlah koloni Streptomyces sp. dan Trichoderma sp. serta daya hambat terhadap jamur Fusarium sp. Hasil penelitian menunjukkan bahwa daya hambat multiantagonis Streptomyces sp. dan Trichoderma sp. terhadap F. oxysporum pada suhu 70 °C di tanah andosol 90% dan terendah pada suhu 70 °C pada tanah regusol 31%.
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