A field experiment was conducted during Kharif, 2021 at crop research farm, Department of Agronomy, SHUATS, Prayagraj (U.P) with the objective to evaluate the influence of molybdenum and biofertilizers on growth and yield of cowpea. The soil texture of the experimental plot was sandy loam, nearly neutral in soil reaction having pH 7.1. The experiment was laid out in Randomized block design with ten treatments and were replicated thrice. The treatments comprising of different levels of molybdenum and bio-fertilizers i.e. Rhizobium and Phosphate solubilizing bacteria whose effect was observed in Cowpea. The treatment 6 with application of molybdenum 4g/kg seed and Rhizobium + PSB recorded significantly higher in plant height (78.64cm), branches/plant (15.07), nodules/plant (37.87) and dry weight (45.11g/plant). Molybdenum 4g/kg seed and Rhizobium + PSB also recorded significantly higher in yield and yield attributes viz. pods/plant (16.20), seeds/pod (12.80), seed yield (1.50t/ha), stover yield (4.17t/ha). Therefore, treatment with application of Molybdenum 4g/kg seed along with Rhizobium and PSB was more productive and can be recommended to farmers after further trails.