Aims:The purpose of the present investigation was to develop a novel method for cell immobilization. Methods and Results: Aureobasidium pullulans cells were mixed with an alginate solution, and the mixture was extruded to form small gel beads as hydrated-immobilized cells. The beads were then placed at )15°C for 6-24 h to induce freeze-dehydration. The freeze-dehydration resulted in shrinkage of beads as a result of water removal reducing bead volume by 82% and bead weight by 85%. The dehydrated beads were successfully used for the production of fructo-oligosaccharides in a model reactor system. Conclusions: Dehydrated beads may provide some commercial advantages over conventional immobilized cells. Significance and Impact of the Study: This study shows that bioreactor performance can be improved up to two times by the use of the dehydrated beads.

Introduction: "Cerebral proliferative angiopathy" (CPA) is a distinct entity unlike "classical" brain arteriovenous malformations (AVMs). Report: We describe a patient with seizure who on DSA showed diffuse angiogenesis, absence of a well formed nidus and early venous phase suggestive of fast capillary transit favouring diagnosis of CPA alongwith filling of ophthalmic artery through middle meningeal branch of external carotid artery which made it even rarer. Treatment options are limited and vary with the patient's presentation and angiographic features. Conclusion: CPA is rare and filling of ophthalmic artery from middle meningeal artery in association with CPA is even rarer.

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