Anemia due to iron deficiency in young girls and pregnant women remains a nutritional health problem in Indonesia. They take iron tablet/day to improve their iron status but it sometimes has some side effects. In Sleman Regency, snake fruits Pondoh are used to make various processed foods but some home food industries leave snake fruit seeds as industrial waste. Therefore, the aim of this study was to extract gradually seeds of snake fruits Pondoh for development of anemia iron supplement. In this explorative study, we collected seeds of snake fruits Pondoh from a home food industry in Turi district, Sleman Regency, Yogyakarta. In brief, we made simplicia of snake fruit seeds in several steps from washing, selecting, drying, cutting in small pieces, grinding to separating with 100 mesh sieve. After that, the simplicia was extracted using tetrahydrofuran, methanol, acetic acid, hexane and or ethyl acetate reagents to separate lipid, water and organic fractions, which were then dried using a vacuum evaporator. Dried fractions of snake fruit seeds (SFS) were finally measured using the inductively coupled plasma or atomic absorption spectroscopy method for iron levels and using ferric chloride for tannin content. From two different methods of making simplicial, we got 26.88 and 22.82% (weigh/weight) iron levels while ethyl acetate SFS fraction had higher iron levels (2.65% w/v) than lipid and water fractions (1.62 and 1.88% respectively). In addition, there was only the water fraction that contained tannin. In conclusion, the ethyl acetate fraction of SFS Pondoh may be useful for development of anemia iron supplement.

In a recent study, snake fruit seed (SFS) flour was found to enhance haemoglobin levels in a rat model of anemia. Still, it increases leucocyte number and its differential count. The purpose of this study was to fractionate SFS Pondoh and to analyze phytochemical compounds in SFS fractions. Simplicia of SFS was separated into lipid, water, and organic fractions using tetrahydrofuran, methanol and ethyl acetate reagents, and then dried using a vacuum evaporator. Chemical components in the SFS fractions were analyzed using gas chromatography-mass spectrophotometry (GC-MS). We got 77.8%, 16.5%, and 7.5% fractions of water, organic, and lipid. The water fraction contained 13 active compounds with 19 to 22 min retention time (RT). The organic fraction also had 13 active compounds with 19 to 29 min RT. The last lipid fraction contained 15 active compounds with 3 to 25 min RT. In conclusion, SFS Pondoh generates the highest water fraction, followed by organic and lipid fractions.