There are few defence mechanisms by which the body identifies and recovers from any physical injuries or external damage-causing stress and stimuli. This is through inflammation wherein the pain and the healing enzymes release into the bloodstream and cause pain and swelling that causes protection from those damages. The most important of the causative factors of the inflammation is the oxidative damage and the physiological stress. There were investigations on the herbs that were used in the treatment of diseases and were claimed to contain very limited or without side effects. Thus, they gained importance these days and were used in the treatment of major diseases like cancer and heart problems. Even though their use was extended from the traditional forms to advanced medicine, their scientific establishment of the proof and validate them for their activities is utmost important. Thus there were gaining interest in the medical and research field to perform the researches on the herbs and medicinal plants to prove their activity. The plant leaves of Pistacia atlantica were extracted with ethanol and water mixture using an ultrasonication. It was tested for the anti-inflammatory potential in various doses like 100, 200, 400 and 600 mg.kg body weight in the swiss albino rats in the cotton pellet induced granuloma method and the extracts showed significant dose-dependent activity compared with the standard indomethacin drug.