The chemical industries currently release pollutants into air, soil, and water. For rubber industry, large amount of wastewater is produced resulting in huge energy demand for water treatment. However, there are several restriction factors for wastewater treatment i. e., contaminated oil from machinery and foul odour from organic matter caused by microbial enzymes. To overcome these problems, this research focused on wastewater treatment from rubber industry via ozonolysis. Two cases of experiments were carried out in a bubble column with capacity of 500 ml including with and without ozonolysis. Ozone concentration were maintained at 64 mg/L. Samples of 10 ml were collected every 4 h and then analyzed by GC- MS. The BOD, COD, and operating cost were investigated. The results showed that the BOD and COD decrease by 40% when ozonolysis was applied. The colour of wastewater become clear after ozonolysis. In addition, the operating cost of ozonolysis process is lower than that of without ozonolysis.