Torula herbarum (Pers.) Link Ex. Fr. is a dermataceous fungus regularly associated with dry leaves and twigs of Felia microphylla Liebm. In submerged cultures the organism produces weakantimicrobial substances whengrownin Czapek's or in a malt extract medium.The temperature range for the growth of the organism was found to be 24~28°C and no growth occurred at 5°or 37°C. Nitrogen and carbon utilization studies indicated tyrosine and levulose to be the best sources, followed by ammonium tartrate and glucose respectively. Based on these studies a synthetic mediumwas developed for the optimum production of pigments. Fermentation Fermentation was carried out in shake flasks (500 ml) and in 5-liter fermentors (New Brunswick) through a seed inoculum at 24°G for 96~120 hours. The fermented broths were extracted at unacidified pH (4.5~5.0) into ethyl acetate and the extracts washed twice with water and concentrated under reduced pressure. Whenthe concentrate was kept overnight at-5°C, a crystalline material separated which varied in

No abstract

No abstract

No abstract

No abstract