The production of β-glucosidase in shake-flasks by Aspergillus wentii

Srivastava, Smita; Gopalkrishnan, K. S.; Ramachandran, K.B.

doi:10.1016/0385-6380(87)90071-9

Cited by 23 publications

(13 citation statements)

References 8 publications

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“…Tween‐80, however, when added after 48 h of fermentation at a concentration of 1 g l −1 , significantly increased the levels of linamarase activity released in the culture medium without affecting fungal growth. Our results seem to confirm the conclusions of Srivastava et al . (1987) that surface‐active agents can cause changes in cell membrane permeability that often result in the increased release of enzymes, particularly cell‐bound enzymes.…”

Section: Discussionsupporting

confidence: 93%

The linamarase ofMucor circinelloidesLU M40 and its detoxifying activity on cassava

Petruccioli

Brimer²,

Cicalini

et al. 1999

Journal of Applied Microbiology

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Mucor circinelloides LU M40 produced 12·2 mU ml−1 of linamarase activity when grown in a 3 l fermenter in the following optimized medium (g l−1 deionized water): pectin, 10·0; (NH4)2SO4, 1·0; KH2PO4, 2·0; Na2HPO4, 0·7; MgSO4.7H2O, 0·5; yeast extract, 1·0; Tween‐80, 1·0, added after 48 h of fermentation. The purified linamarase was a dimeric protein with a molecular mass of 210 kDa; the enzyme showed optimum catalytic activity at pH 5·5 and 40 °C and had a wide range (3·0–7·0) of pH stability. The enzyme substrate specificity on plant cyanogenic glycosides was wide; the Km value for linamarin was 2·93 mmol l−1. The addition, before processing, of the fungal crude enzyme to cassava roots facilitated and shortened detoxification; after 24 h of fermentation, all cyanogenic glycosides were hydrolysed.

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Section: Discussionsupporting

confidence: 93%

The linamarase ofMucor circinelloidesLU M40 and its detoxifying activity on cassava

Petruccioli

Brimer²,

Cicalini

et al. 1999

Journal of Applied Microbiology

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“…For B. tequilensis HMF6X, the maximum SSF-processing of LOC. An inoculum size varying between 4-6% has been described as optimum for cellulase production by diverse microorganisms [65], which was close to our observation. Aspergillus flavus AT-2 and Aspergillus niger AT-3 revealed maximum cellulase production under SSF with an inoculum size of 5% [66].…”

Section: Effect Of Inoculum Sizesupporting

confidence: 79%

Bio-processing of Linseed Oil-Cake through Solid State Fermentation by Non- Starch Polysaccharide Degrading Fish Gut Bacteria

Banerjee¹,

Ghosh²

2016

Ferment Technol

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Cellulose and hemicellulose (xylan) are the most common Non-Starch Polysaccharides (NSPs) present in plant ingredients that exhibit anti-nutritional effect. Degradation of cellulose and xylan has been investigated under solidstate fermentation (SSF) using oil-cakes, viz., groundnut oil-cake (GOC), mustard oil-cake (MOC), sunflower oilcake (SOC), sesame oil-cake (SeOC) and linseed oil-cake (LOC) as substrates. Finally LOC was considered in the present study as it contained maximum amount of cellulose (17.51 ± 0.87 g 100 g -1 ) and xylan (13.02 ± 0.66 g 100 g-1 ). Bio-processing of LOC was carried out through SSF in two different combinations utilizing efficient cellulaseand xylanase-producing strains, Bacillus pumilus KF640221 (Set-I) and Bacillus tequilensis KF640219 (Set-II), isolated previously from the proximal intestines of rohu, Labeo rohita and silver carp, Hypophthalmichthys molitrix, respectively. Analysis of the fermentation sets revealed that B. pumilus KF640221 in Set-I was most effective in reducing the cellulose and xylan contents of LOC. Besides, SSF under optimized conditions caused considerable increase in crude protein, crude lipid, total free amino acids and total free fatty acids along with reduction in the contents of other anti-nutritional factors, e.g., crude fiber, tannins, phytic acid and trypsin inhibitor. High performance liquid chromatography (HPLC) analysis of raw and fermented LOC of Set-I indicated that concentrations of methionine, histidine, tryptophan, phenylalanine, threonine were increased considerably. Further research is inevitable to explore the possibilities for utilization of SSF-processed LOC to set up a strategy for sustainable utilization of low cost oil-cakes as animal feed ingredients.

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“…It is noted that higher titer and productivity could be obtained with further process optimization and different organisms such as the marine Aspergillus strain SA 58 [9]. Although higher β-glucosidase A. wentii 3% Cellulose 10 1.25 [16] Aspergillus SA 58 Pectin 80 20 [9] Fusarium oxysporum Papyrus paper 2.32 0.116 [10] Trichoderma virdie 1% Bagasse + 1% Bran 0.261 0.016 [15] production has been reported from xylan [13], malt extract [17], and pectin [9] (see Table 5), those processes were not economical because of the expensive substrates used in the fermentation. In our study, we achieved high β-glucosidase production from low-cost agricultural residue wheat bran and glycerol, an abundant byproduct from biodiesel production.…”

Section: Discussionmentioning

confidence: 99%

“…This might be due to poor cell growth, perhaps because of a lack of adequate endo-and exo-glucanases to break down cellulose to soluble sugars. Compared to cellobiose, β-glucosidase production from glucose (3.60 IU/ml) and sucrose (3.62 IU/ml) was only slightly lower, indicating that the enzyme is constitutive in A. niger [17]. The somewhat lower enzyme production from glucose could be attributed to glucose being a catabolite repressor and inhibitor to cellulases [25].…”

Section: Effect Of Carbon Source On β-Glucosidase Production Bymentioning

confidence: 94%

Production of β-Glucosidase by Aspergillus niger on Wheat Bran and Glycerol in Submerged Culture: Factorial Experimental Design and Process Optimization

Abdella¹,

Mazeed²,

Yang³

et al. 2014

CBIOT

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The goal of this study was to develop a fermentation process for the production of β-glucosidase, an important enzyme in the hydrolysis of lignocellulose and has many applications in food and flavor industries, using low-cost agricultural residues as substrates. Based on statistical experimental design, high-titer production of β-glucosidase on wheat bran and glycerol by Aspergillus niger in a submerged culture was achieved. A 2-level Plackett-Burman design was first used to screen the bioprocess parameters affecting β-glucosidase production. Among the tested parameters, the concentrations of wheat bran, glycerol, corn steep liquor and KCl showed significant effects on β-glucosidase production. These four medium components were further optimized using a 3-level Box-Behnken design, and their optimal levels were found to be: wheat bran, 3.5 g/L; glycerol, 5 g/L; KCl, 0.1 g/L and corn steep liquor, 7.5 g/L, giving a high βglucosidase titer of 9.37 IU/ml, 1.6-fold of the maximum level obtained in the screening experiment. With the optimized medium, 5.41 IU/ml of β-glucosidase was produced in a stirred-tank bioreactor inoculated with a spore suspension. When the reactor was inoculated with precultured cell pellets and operated at optimized agitation (450 rpm) and aeration rates (2 vvm), β-glucosidase production increased to 9.33 IU/ml, which was comparable to the level obtained in shake-flasks. This study demonstrated a scalable fermentation process for the production of β-glucosidase on low-cost wheat bran and glycerol, which should have important industrial applications.

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The production of β-glucosidase in shake-flasks by Aspergillus wentii

Cited by 23 publications

References 8 publications

The linamarase ofMucor circinelloidesLU M40 and its detoxifying activity on cassava

The linamarase ofMucor circinelloidesLU M40 and its detoxifying activity on cassava

Bio-processing of Linseed Oil-Cake through Solid State Fermentation by Non- Starch Polysaccharide Degrading Fish Gut Bacteria

Production of β-Glucosidase by Aspergillus niger on Wheat Bran and Glycerol in Submerged Culture: Factorial Experimental Design and Process Optimization

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The production of β-glucosidase in shake-flasks by Aspergillus wentii

Cited by 23 publications

References 8 publications

The linamarase ofMucor circinelloidesLU M40 and its detoxifying activity on cassava

The linamarase ofMucor circinelloidesLU M40 and its detoxifying activity on cassava

Bio-processing of Linseed Oil-Cake through Solid State Fermentation by Non- Starch Polysaccharide Degrading Fish Gut Bacteria

Production of &#946;-Glucosidase by Aspergillus niger on Wheat Bran and Glycerol in Submerged Culture: Factorial Experimental Design and Process Optimization

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Production of β-Glucosidase by Aspergillus niger on Wheat Bran and Glycerol in Submerged Culture: Factorial Experimental Design and Process Optimization