No abstract

The World Organization for Animal Health (OIE) Code chapter on FMD includes camelids as being susceptible species to FMD similar to cattle, sheep, goats and pigs. A total of 376 field camel sera, collected from different regions of Riyadh and AlQassim Province in the Kingdom of Saudi Arabia, were screened for the presence of antibodies produced against 3ABC non-® structural proteins (NSP) of FMDV using a commercially available kit , PrioCHECK FMDV NS. Sera that tested positive on NSP were screened for serotype-specific antibodies towards the seven serotypes of FMD virus using liquid phase blocking ELISA. Only 24 out of 376 (6.3%) serum samples were positive for antibodies against NSP. All sera that tested positive on NSP and screened for antibodies against all the seven FMDV serotypes (O, A, C, Asia 1, SAT 1, SAT 2 and SAT 3) were found positive for antibodies against serotype O. This reveals that dromedaries appear however as being susceptible to infection with FMDV serotype O, but they are unlikely to play any significant role in the natural epidemiology of FMD.

‫أبوسبعين‬ ‫الذرة‬ ‫علف‬ ‫مع‬ ‫بالمقارنة‬ ‫العلف‬ ‫لبنجر‬ ‫الغذائية‬ ‫القيمة‬ ‫السودانية‬ ‫الظروف‬ ‫تحت‬ ‫الحاج‬ ‫محجوب‬ ، ‫دقش‬ ‫ياسين‬ ، ‫خوجلى‬ ‫الطيب‬ ‫منى‬ cross-bred (Frisian X Kenana) milking cows and their impact on feed intake, milk yield, milk composition and economics of feeding was examined. Neither milk yield (8.65Kg vrsus8.67) nor its composition [fat (3.55% versus3.45%), lactose (4.52% versus 4.56%) and solids-non fat(8.54% versus8.57%)] showed significant differences (p>0.05) between cows fed on beet and those fed on Abu 70. The nutritive value of beet forage was better than Abu70 in terms of low CF content, high amounts of NFE (carbohydrates), CP and ME. The feeding value of beet forage was by far superior to Abu 70 as reflected in the lower DM intake and equal or superior yields of milk by cows fed on beet without any adverse effects on milk quality. Feeding costs decreased significantly along the two seasons by > 30% when cows fed on fodder beet.

75 camels (12%) as positive for the presence of T. evansi. While, Out of 75 DNA samples prepared from camel blood, 72 were positive by real time PCR assay (96%). Primers (TR3, TR4) derived from nuclear repetitive gene of T.evansi and SYBER Green1 fluorescent dye has been used. The melting peak chart of the positive samples showed one single peak at an average Tm of 85.0 o C. Application of this real time PCR to clinical samples resulted in rapid, direct detection of T. evansi in blood of naturally infected camels. The described real time PCR provides a valuable tool to study the epidemiology of T.evansi infection in camels and other susceptible animal population.