An isocratic, reversed phase-liquid-chromatographic method was developed for the quantitative determination of Levofloxacin and Cefpodoxime proxetil in combined-dosage form. Alliance -Waters System with Agilant Zorbax Eclipse XBD-C8, (150mm×4.6; 5µm) column with mobile phase containing water with Ortho phosphoric acid: Methanol in the ratio of (80: 20, v/v) was used. The flow rate was 0.5 ml/min, column temperature was 40°C and effluents were monitored at 270 nm. The retention times of Levofloxacin and Cefpodoxime proxetil were 3.096min and 4.559min, respectively. The correlation co-efficient for Levofloxacin and Cefpodoxime proxetil was found to be 1.0 and 1.0, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Levofloxacin and Cefpodoxime proxetil in formulations was found to be in the range of 97-103% and 97-103% respectively confirms the non-interferences of the excipients in the formulation. Due to its simplicity, rapidness and high precision. The method was successfully applied for the estimation of Levofloxacin and Cefpodoxime proxetil in combined dosage form.

Objective: The focus of our research was to create a fairly sensitive HPLC stratagem for determining telmisartan (TLM) and azelnidipine (AEL) in bulk and tablet types. Methods: Analysis of TLM and AEL was performed on a “C18 Kromasil stationary column (5 µm, 250 mm × 4.6 mm)”. The mobile phase was made of 0.1M NaH2PO4 solution (pH 3.5) and methanol at a comparative volume ratio of 50% each. The analysis of TLM and AEL was isocratic, with the flow velocity adjusted at 1.0 ml/min and indeed, the TLM and AEL analysis was done at 256 nm using a PDA device sensor. TLM and AEL were stressed with acid, peroxide, dry heat, alkali, and sunlight-induced settings. Results: The retention/elution periods for the TLM and AEL were observed at 2.225 min and 3.178 min, respectively. The HPLC stratagem developed have a straight-line relation with relative concentrations in the ranges of 20-60 µg/ml for TLM and 4-12 µg/ml for AEL. The LOQ’s for TLM and AEL were 0.2516 μg/ml and 0.0871 μg/ml, respectively. The validation investigational findings done for TLM and AEL with the established sensitive HPLC stratagem were passed out in conformity with the ICH standards. Conclusion: The established sensitive HPLC stratagem was shown as competent for the quality check of bulk samples of TLM and AEL throughout batch release as well as in the course of TLM and AEL stability investigations.

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