Serrapeptase is an anti-inflammatory, proteolytic enzyme isolated from the microorganism, Serratia sp. HY-6. Very few methods are available for the quantification of serrapeptase. The activity of the enzyme is determined by an ELISA assay, colorimetric method using casein as substrate or by HPLC method. These methods are lengthy, time consuming and require a number of reagents and solvents. Therefore an attempt was made to develop a simple alternative method for regular estimation of drug in formulations. Serrapeptase enzyme was estimated in formulations by using microplate readers which uses the principle of vertical photometry. Further this method was validated and the robustness of this method was checked by estimating the drug in various formulations including liposomes and marketed tablet formulations. A linear relationship between drug concentration and absorbance was observed between 1-4 microg/ml at 230 nm (R(2)=0.9911). The percentage recovery values of the drug in serrapeptase liposomes were found to lie within the standard limit (97-98%) which confirms the method is accurate and free from any positive or negative interference of the excipient. The low value of standard deviation obtained confirms the precision of the method. (+/-0.020 - +/-0.044). The drug content values in marketed tablets values obtained matched the label claim. The proposed microplate UV-method for determination of serrapeptase in formulations is novel, simple, inexpensive, fast, specific and robust. Thus this method could be a better alternative for regular estimation of drug in the various marketed formulations of serrapeptase.

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Diabetes Mellitus is a chronic metabolic disorder affecting the blood glucose levels in both the young and old. The impaired glucose levels if not carefully monitored in the blood affects multiple systems in the body leading to further complications like loss of vision and limbs, failure of important organs like the kidney, pancreas etc., Regular monitoring of blood glucose levels of patients who pose higher risk of damage to the eyes, kidneys can facilitate early treatment. Most of the present methods used to monitor blood glucose levels are invasive techniques, which require a single drop of blood preferably from the fingertip that causes a lot of inconvenience and discomfort to diabetic patients who require blood glucose to be monitored 4–5 times a day. Since continuous glucose, monitoring is critical and to overcome the disadvantages posed by the current invasive techniques, the present study aims to develop non-invasive colorimetric detection of glucose using paper based medium in saliva.

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