DNA sequencing methods were first developed more than 20 years ago with the publication of two approaches to sequencing methodology that became known as Sanger sequencing (1), based on enzymatic synthesis from a single-stranded DNA template with chain termination using dideoxynucleotides (ddNTPs) and Maxim-Gilbert sequencing (2),which involved chemical degradation ofend-radio-labeled DNA fragments. Both methods relied on four-lane,high resolution polyacrylamide gel electrophoresis to separate the labeled fragment and allow the base sequence to be read in a staggered ladder-like fashion. Sanger sequencing was technically easier and faster, and thus became the main DNA sequencing method for the vast majority of applications.

This research aimed to produce a device for converting heat energy into electrical energy as a teaching aid for physics. This research process is development research, which refers to the Research and Development (R&D) model. The instrument used was a questionnaire with a five Likert scale, which addressed media experts, material experts, physics teachers in SMA Negeri 2 Sigi, and class XII MIA SMA Negeri 2 Sigi students. The product quality data obtained were analyzed using descriptive analysis. Based on the analysis of the media expert’s assessment, the quality of the product obtained a percentage value of 87.69% with the interpretation of “Very Good”. Based on the analysis of the assessment by material experts, the quality of the product obtained a percentage value of 84.44% with the interpretation “Very Good”. For the results of the physics teacher’s response, a percentage value of 86.00% was obtained with the interpretation “Strongly Agree”. For the results of the responses of class XII MIA students in the limited test, a percentage value of 83.56% was obtained with the interpretation “Strongly Agree”. The test results and questionnaire analysis showed that the heat energy conversion device developed into electrical energy was feasible to use.