The culture filtrate of Bacillus subtilis strain C2 showed strong activity against the pathogenic fungus Fusarium solani f. sp. radicicola. A partially purified fraction (PPF) from the extract induced chlamydospore formation in Fusarium. Reverse-phase high performance liquid chromatography yielded 8 different fractions, six of which had chlamydospore-inducing activity. Mass spectrometry and nuclear magnetic resonance analyses identified the main active constituent as C(17) fengycin A (FA17), a cyclic lipopeptide. The effect of FA17 on morphology and physiology of two Fusarium species was dependent on the lipopeptide concentration. When challenged with FA17 at concentrations (0.5, 8, 64 μg ml(-1)) below the minimum inhibitory concentration (MIC) (128 μg ml(-1)), two species of Fusarium formed chlamydospores from hyphae, germ tubes, or inside the conidia within 2 days. At concentrations close to the MIC, FA17 caused Fusarium to form sparse and swollen hyphae or lysed conidia. The other five fractions were identified as fengycin A homologues. The homologues could also induce chlamydospore-like structures in 17 species of filamentous fungi including some specimens that do not normally produce chlamydospores, according to their taxonomic descriptions. Like other chlamydospores, these structures contained nuclei and lipid bodies as revealed by DAPI and Nile Red staining, and could germinate. This is the first study to demonstrate that under laboratory conditions fengycin, an antifungal lipopeptide produced by B. subtilis, can induce chlamydospore formation in Fusarium and chlamydospore-like structures in many filamentous fungi.

Three soil bacterial strains were identified as Chryseobacterium sp. TFB on the basis of their 16S rRNA gene sequences. Conidia of Arthrobotrys oligospora produced a few mycelial traps (MT) and conidial traps (CT) when cultured with bacterial cells that they did not produce when cultured with a bacterial cell-free culture filtrate. However, co-culture of A. oligospora with bacterial cells and bacteria-free filtrate simultaneously induced MT and CT in large amounts. With the increased concentration of bacteria-free filtrate, the number of typical CT increased, but conidial germination was progressively inhibited. Scanning electron microscopy of A. oligospora co-cultured with bacteria revealed that bacterial attachment to hyphae was a prerequisite to trap formation and that bacteria-free filtrate facilitated bacterial attachments to hyphae. The results that the addition of nutrients in co-culture medium decreased the number of traps suggest that this type of trap formation may be favoured at a low nutrient status. Eight fungi tested were able to form MT and CT when co-cultured with bacterial cells and bacteria-free culture filtrate, but the abilities varied among species. This study provides novel evidence that under laboratory conditions, soil bacteria attaching to hyphae could induce traps in nematode-trapping fungi.

An anamorphic fungus was isolated from fresh specimens of Orbilia species collected in Yunnan Province, China. The fungus was characterized by very short conidiophores and 1-5 septate cylindrical conidia, and most closely related phylogenetically to Dactylella vermiformis that produces branched conidiophores and 0-1 septate conidia, but the calculated similarity of ITS sequences was only 83% between the two fungus species. Considering the morphological characteristics and the calculated similarity value, a new species, Dactylella pseudobrevistipitata, was described with holotype YMF 1.03504.

Sympodioplanus yunnanensis sp. nov. from submerged leaves collected from Gengma county, Lincang city, was found when researching aquatic hyphomycetes in Yunnan. The new species is well defined as a Sympodioplanus by its sympodial proliferation with multiple and crowded conidiogenous loci in the upper conidiophores and elongated conidia. It differs in conidial shape and septation from the type species, S. capensis.

BACKGROUND Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world, which is seriously threatening the lives of patients. Due to the rapid development of the disease, patients were in the middle and advanced stages at the time of diagnosis and missed the best time for treatment. With the development of minimally invasive medicine, interventional therapy for advanced HCC has achieved promising results. Transarterial chemoembolization (TACE) and transarterial radioembolization (TARE) are currently recognized as effective treatments. This study aimed to investigate the clinical value and safety of TACE alone and combined with TACE in the treatment of progression in patients with advanced HCC and to find a breakthrough for the early diagnosis and treatment of patients with advanced HCC. AIM To investigate the efficacy and safety of hepatic TACE and TARE in advanced descending hepatectomy. METHODS In this study, 218 patients with advanced HCC who were treated in the Zhejiang Provincial People’s Hospital from May 2016 to May 2021 were collected. Of the patients, 119 served as the control group and received hepatic TACE, 99 served as the observation group and were treated with hepatic TACE combined with TARE. The patients in two groups were compared in terms of lesion inactivation, tumor nodule size, lipiodol deposition, serum alpha-fetoprotein (AFP) level in different periods, postoperative complications, 1-year survival rate, and clinical symptoms such as liver pain, fatigue, and abdominal distension, and adverse reactions such as nausea and vomiting. RESULTS The observation group and the control group had good efficacy in treatment efficiency, reduction of tumor nodules, reduction of postoperative AFP value, reduction of postoperative complications, and relief of clinical symptoms. In addition, compared with the control group, the treatment efficiency, reduction of tumor nodules, reduction of AFP value, reduction of postoperative complications, and relief of clinical symptoms in the observation group were better than those in the TACE group alone. Patients in the TACE + TARE group had a higher 1-year survival rate after surgery, lipiodol deposition was significantly increased and the extent of tumor necrosis was expanded. The overall incidence of adverse reactions in the TACE + TARE group was lower than that in the TACE group, and the difference had statistical significance ( P < 0.05). CONCLUSION Compared with TACE alone, TACE combined with TARE is more effective in the treatment of patients with advanced HCC. It also improves postoperative survival rate, reduces adverse effects, and has a better safety profile.