Mutations in the PARK2 (parkin) gene are responsible for an autosomal recessive form of Parkinson's disease. The parkin protein is a RING-in-between-RING E3 ubiquitin ligase that exhibits low basal activity. We describe the crystal structure of full-length rat parkin. The structure shows parkin in an autoinhibited state and provides insight into how it is activated. RING0 occludes the ubiquitin acceptor site Cys(431) in RING2, whereas a repressor element of parkin binds RING1 and blocks its E2-binding site. Mutations that disrupted these inhibitory interactions activated parkin both in vitro and in cells. Parkin is neuroprotective, and these findings may provide a structural and mechanistic framework for enhancing parkin activity.
This paper introduces a novel eigenstructure-based algorithm uni-vector-sensor ESPRIT that yields closed-form direction-of-arrival (DOA) estimates and polarization estimates using one electromagnetic vector sensor. A vector sensor is composed of six spatially co-located nonisotropic polarizationsensitive antennas, measuring all six electromagnetic field components of the incident wave field. Uni-vector-sensor ESPRIT is based on a matrix-pencil pair of temporally displaced data sets collected from a single electromagnetic vector sensor. The closed-form parameter estimates are obtained through a vector cross-product operation on each decoupled signalsubspace eigenvector of the data correlation matrix. This method exploits the electromagnetic sources' polarization diversity in addition to their spatial diversity, requires no a priori knowledge of signal frequencies, suffers no frequency-DOA ambiguity, pairs automatically the x-axis direction cosines with the y-axis direction cosines, eliminates array interelement calibration, can resolve up to five completely polarized uncorrelated monochromatic sources from near field or far field. It impressively out-performs an array of spatially displaced identically polarized antennas of comparable array-manifold size and computational load.
Abstract-A sparse uniform Cartesian-grid array suffers cyclic ambiguity in its Cartesian direction-cosine estimates due to the spatial Nyquist sampling theorem. The proposed MUSIC-based or MODE-based algorithm improves and generalizes previous disambiguation schemes that populate the thin array grid with identical subarrays-such as electromagnetic vector sensors, underwater acoustic vector hydrophones, or half-wavelength spaced subarrays.Index Terms-Antenna arrays, array signal processing, direction-of-arrival estimation, electromagnetic wave polarization, intelligent sensors, land mobile radio diversity systems, radar signal processing, radio direction finding.
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