<p class="Abstract">The core objective of this study was to investigate the in vitro anti-oxidant and antihyperglycemic effect of the ethanol extract of <em>Parmotrema </em>hababianum in streptozocin-induced diabetic rats for 42 days. The extract showed nearly all anti-oxidant activities with maximum presence. The treatment with extract in diabetic rats at the dosage level of 100 and 200 mg/kg, which is compared with diabetic control and glibenclamide at a dosage level of 4 mg/kg and the biochemical parameters such as blood glucose, total cholesterol, triglycerides, HDL, LDL, insulin, total protein, urea and creatinine were assessed. The extract showed positive correlation (p<0.001) in reducing blood glucose level as compared to the control. Moreover, there was a significant (p<0.01) decrease in TC, TG, LDL, SGPT, SGOT, urea and creatinine level and significant (p<0.01) increase in HDL and insulin level. Thus, the results of this study show considerable efficacy in curing diabetes with potent anti-oxidant and anti-hyperglycemic activity.</p><p class="Abstract"><strong>Video Clip of</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/Lw2vvLpKNTE">Methodology</a>: 6 min 12 sec</p>
The silver nanoparticles (AgNP's) were synthesized using green synthesis and chemical synthesis method. The nanoparticles synthesized using green synthesis (boiled extract and normal extract) were compared for the difference in various properties after characterization using X-ray diffraction (XRD), Fourier Transform Infra-Red spectroscopy (FT-IR) and Atomic Force Microscopy (AFM). There is not much difference between the AgNP's synthesized using both the methods. The chemically synthesized AgNP's were also characterized. FTIR analysis revealed that there is a strong peak of absorbance at 1379 cm -1 on comparison with the control. XRD analysis is used to find the nature and size of the silver nanoparticle. The silver nanoparticles are found to be more stable in distilled water than the deionized water and tap water. The antimicrobial activity is high for the boiled extract sample when compared with other samples. The effect of AgNP's on Vigna radiata is studied by exposing the seeds in time intervals and allowing them to germinate. The comparison was done by estimation of chlorophyll and protein. Based on the studies it could be said that if the exposure to silver nanoparticles increases the content of the plant will be affected.
Oxidative stress is believed to be a key factor in the pathogenesis and perpetuation of the mucosal damage in inflammatory bowel disease. The investigation was envisaged to assess the protective effect of diethyl ether extract of leaves of Aegle marmelos Corr. (DEAM) in view of reported anti-inflammatory and antioxidant properties in acetic acid-induced enterocolitis in albino Wistar rats. Enterocolitis was induced by intrarectal injection of acetic acid (5% V/V). The study comprised of five different groups consisting of six animals each and two doses [100, 200 mg / kg] were selected. The protective effect in the treatment group of animals was assessed by measuring levels of biomarkers of inflammation [MPO] and oxidative stress [CAT, SOD and GSH] in the inflamed colon and several physiological parameters. Significant and dose dependent changes in the levels of biomarkers of oxidative stress in DEAM treated animals was suggestive of the antioxidant-mechanism protective effect by Aegle marmelos.
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