Biochemical profiles of chickens provide valuable information for evaluation of health status and are reliable indicator of health. The sole purpose of the present study was to determine and compare the base line value of certain serum biochemical profile of broiler and indigenous chickens of Assam. Blood samples from apparently healthy, 20 each broiler (cob 400 strain) and indigenous chickens of Assam weighing between 1.5-2.0Kg were collected from local butchers at the time of slaughter, early in the morning in three different days from Guwahati city and average values for each parameters were taken. Sodium fluoride was used as anticoagulant for estimation of blood glucose and other parameters namely total protein, albumin, globulin, cholesterol, urea, uric acid and creatinine was estimated in serum following standard protocol. Data generated from the experiment were analyzed statistically using SAS Enterprise Guide (Version 4.2). From the present study, it was concluded that certain parameters like glucose, total protein, albumin, globulin, urea, uric acid and cholesterol significantly (P<0.05) varied between broiler and indigenous chickens of Assam. However, the concentration of creatinine did not differed between the two groups.