BACKGROUND Menstrual cycle is the cyclical phenomenon characterized by periodic vaginal bleeding. It influences all the system of the body. Menstruation occurs cyclically at intervals of 21-35 days. Uterine cycle includes menstruation, proliferative and secretory phases. It is due to invisible interplay of different levels of hormones mainly through hypothalamo-pituitary-ovarian-axis. The physiological levels of oestrogen and progesterone influence the leucocyte count. Thus, change in the count might occur when the hormonal balance changes during menstrual cycle and most commonly the organs in the body remain unaffected by this hormonal imbalance. We wanted to study the age-related variations in total leucocyte, granulocyte and agranulocyte count during different phases of menstrual cycle in 1 st professional MBBS students at, Jawaharlal Nehru Institute of Medical sciences (JNIMS), Imphal, Manipur.
METHODSIt is a longitudinal study. 50 healthy female medical students in the age group of 18-25 yrs. with regular cycles of 28±2 days duration participated in the study. Students with irregular cycles, gynaecological disorders like endometriosis, fibroid, irregular bleeding, history of prolonged drug intake and history of fever were excluded from the study. Study protocol was explained to the students and informed consent was obtained from each of the students. Institutional ethical committee clearance was obtained. First sample was collected within 48 hours of onset of menstruation, second sample during 8 th -10 th day of menstrual cycle. Third sample was taken during 22 nd -24 th day of menstrual cycle. All the subjects were followed up during a single cycle. Samples were taken in the morning between 9 am-10 am to avoid diurnal variation. Total leucocyte count was determined with Improved Neubauer's chamber using Turk's fluid. Granulocyte-and agranulocyte-count was determined using Leishman's stain under compound microscope. Total leukocyte count and granulocyte and agranulocyte cells were examined during a single cycle. Data collected was statistically analysed.
RESULTSThere was increase in total leukocyte count in the 23-25 years age group in different phases of the menstrual cycle as compared to other age groups but was not statistically significant and increased granulocyte count was seen in both the aged groups (17-19), (23-25) years in all phases of menstrual cycle was statistically significant. Agranulocyte count was increased in 23-25 years age group in all phases but was not statistically significant.
Introduction: Childbirth is often delayed in the contemporary world in view of advancing profession and social reasons. This has lead to increasing infertility. And this also has brought in many studies on age and ovarian reserves even in India. However the northeastern part of India is demographically and geographically different from the rest of India and similar to only a small part of the world population. This study was conducted to find if the above mention factors has any role in the relation of age on ovarian reserve and also with the ABO blood grouping. Material and methods: It was an observational clinical study with a duration of 3 years on patients with infertility. Descriptive and inferential statistical analysis was carried out in the present study. Result: 80% of the study population, irrespective of the age group and blood group has basal FSH < 10mIU/ml. However correlation statistics of age with bFSH shows age (in years) of 35.02±3.13 with bFSH >10mIU/ml and have p value of 0.020. Blood group O shows FSH > 10mIU/ml and blood group A,B,AB does not manifest any relation with FSH. Conclusion: Age has directly related with ovarian reserve represented by basal FSH, and blood group O too has direct correlation with impaired ovarian reserve.
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