RIV4 provided better protection than standard-dose IIV4 against confirmed influenza-like illness among older adults. (Funded by Protein Sciences; ClinicalTrials.gov number, NCT02285998 .).

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Immunofluorescence microscopy of cultured animal cells is often performed after detergent permeabilization of formaldehyde-fixed cellular membranes so that antibodies may have access to intracellular antigens. A comparison was made of the ability of several detergents, after formaldehyde fixation, to affect localization of intracellular proteins or to permeabilize different organelles to antibodies. Saponin, a detergent-like molecule that can permeabilize cholesterol-containing membranes, was also used. Four monoclonal antibodies were found to have a bright, discrete fluorescence localization with saponin alone, but were almost undetectable when the cells were treated with nonionic detergents such as Triton X-l00 or NP-40. These immunoglobulin G antibodies included two against lysosomal membrane glycoproteins, one against an integral GOLDENTHAL, HEDMAN, CHEN, AUGUST, WILLINGHAM Materials and Methods Cell cultures. Four cell lines were examined: fibroblastic mouse NIH 3T3 and Swiss 3T3 cells, baby hamster kidney (BHK) cells, and ' + 3, discrete and very bright;

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