Of 108 lactic acid bacteria (LAB) isolates, 9 isolates had a broad antifungal activity against Penicillium Spp. were isolated from naturally fermented milk products. The isolates were processed under cheese manufacturing conditions to study its relation with prolonging the shelf-life of cheese. The technological properties of these isolates has been determined by studying the acidifying and proteolysis activity. Three isolates with moderate or low acidifying and proteolysis activity were selected for further studies. The factors affecting the rate of growth of these selected strains, i.e. pH. NaCl and temperature were evaluated. The results indicated that, strains grown well at pH 5.5-6.6, tolerate salt concentration at 3-5% and showed good growth rate at 10 and 37ᴼC. These properties gave these strains potentiality for use as starter-like or as adjunct cultures. When these strains used as adjunct culture in Ras cheese manufacture, the cheeses had composition resembling normal composition of Ras cheese with positive effect on cheese quality. The cheeses had prolonged shelflife against fungi spoilage.