The present study was undertaken to evaluate and compare the potential of antioxidant activities of olive leaf extracts(OLEs) with other natural or synthetic antioxidants. So, the aim of this study was to assess the effectiveness of methanolic-,ethanolic-,and water-olive leave extracts (OLEs) and compare it with other antioxidants such as green tea extract , tert-butylhydroxyquinone (TBHQ) , citric acid on the oxidative stability of virgin canola oil during storage at an oven at 63±1°C temperature for 0,5,10,15,20 days. To follow the relative of oxidative deterioration of canola oil , oils were analyzed periodically for their peroxide value (PV) , iodine value (IV) , acid value (AV) and Thiobarbituric acid (T.B.A.). Analysis of heated canola oils demonstrated significant increases in PV and AV and T.B.A. However , IV of the oils were markedly decreased. Results also showed that olive leaf extracts (i.e.,methanolic-,ethanolic-,and water-olive leave extracts) secured protective effect against oxidation of canola oil and can serve as substitutes for synthetic antioxidants .

Background: The role of surface contamination in infections is of interest in healthcare as well as other industries, especially where infections incur high cost. One such industry is professional sports, where infections pose significant risks to players and the organizations that employ them. Sports training facilities experience highly variable occupancy rates due to differing seasonal activities, presenting a measurement challenge because the relationship between occupancy and surface contamination is not well described. In a recent publication, a continuously active antimicrobial (CAA) surface coating demonstrated a reduction in bacterial bioburden in ICUs alongside a reduction in healthcare-associated infections (HAIs). Objective: We investigated the impact of a CAA surface coating on bioburden in 2 professional sports training facilities, despite changes in occupancy. Methods: A CAA surface coating was applied using an electrostatic sprayer to all surfaces in both facilities during a period of high-occupancy at facility A and during low occupancy at facility B. Surface cultures were taken using 3M Sponge-Sticks from lockers, gym equipment, and physiotherapy surfaces before treatment, 4–13 weeks after treatment at facility A and 4–23 weeks after treatment at facility B. Total aerobic bacteria counts were obtained by plating on tryptic soy agar, and geometric means of aerobic plate counts (APCs) were used to compare bioburden before and after treatment at both facilities and for an out-of-efficacy period at facility B (17–23 weeks). Occupancy rates were monitored as person days per week (pd/w) over the course of the study. Results: APC counts at facility A decreased 61% (585 CFU/100 cm2 to 226 CFU/100 cm2) from baseline to posttreatment, and occupancy remained constant (165 pd/w to 171 pd/w). At facility B, there was no significant change in APC (76 CFU/100 cm2 to 80 CFU/100 cm2), although occupancy increased >13,000% during the treatment period (3 pd/w to 386 pd/w). During the out-of-efficacy period at facility B, total bacteria increased 170% (217 CFU/100 cm2) compared to the treatment period, and the occupancy remained relatively constant (344 pd/w). Conclusions: Levels of bioburden were significantly influenced by the application of the CAA surface coating, especially considering the variation in occupancy in both facilities before, during, and after the efficacy period. Facility A saw a significant reduction in bioburden during the treatment period (P < .0001), and a significant increase was observed at facility B during the out-of-efficacy period (P < .0001) despite constant occupancy rates, demonstrating the ability of the surface coating to reduce bioburden levels despite large changes in occupancy.Funding: Allied BioScience, Inc, provided Funding: for this study.Disclosures: Gavri Grossman, Valerie Beck, and Daniel S Watson report salary from Allied BioScience.

Of 108 lactic acid bacteria (LAB) isolates, 9 isolates had a broad antifungal activity against Penicillium Spp. were isolated from naturally fermented milk products. The isolates were processed under cheese manufacturing conditions to study its relation with prolonging the shelf-life of cheese. The technological properties of these isolates has been determined by studying the acidifying and proteolysis activity. Three isolates with moderate or low acidifying and proteolysis activity were selected for further studies. The factors affecting the rate of growth of these selected strains, i.e. pH. NaCl and temperature were evaluated. The results indicated that, strains grown well at pH 5.5-6.6, tolerate salt concentration at 3-5% and showed good growth rate at 10 and 37ᴼC. These properties gave these strains potentiality for use as starter-like or as adjunct cultures. When these strains used as adjunct culture in Ras cheese manufacture, the cheeses had composition resembling normal composition of Ras cheese with positive effect on cheese quality. The cheeses had prolonged shelflife against fungi spoilage.