Abstract:Biological hazardous substances such as certain fungi and bacteria represent a high risk for the broad public if fallen into wrong hands. Incidents based on bio-agents are commonly considered to have unpredictable and complex consequences for first responders and people. The impact of such an event can be minimized by an early and fast detection of hazards. The presented approach is based on optical standoff detection applying laser-induced fluorescence (LIF) on bacteria. The LIF bio-detector has been designed for outdoor operation at standoff distances from 20 m up to more than 100 m. The detector acquires LIF spectral data for two different excitation wavelengths (280 and 355 nm) which can be used to classify suspicious samples. A correlation analysis and spectral classification by a decision tree is used to discriminate between the measured samples. In order to demonstrate the capabilities of the system, suspensions of the low-risk and non-pathogenic bacteria Bacillus thuringiensis, Bacillus atrophaeus, Bacillus subtilis, Brevibacillus brevis, Micrococcus luteus, Oligella urethralis, Paenibacillus polymyxa and Escherichia coli (K12) have been investigated with the system, resulting in a discrimination accuracy of about 90%.

A rectangular negative branch off-axis hybrid resonator was coupled to a 10 kW class chemical oxygen-iodine laser. Resonator setup and alignment turned out to be straightforward. The extracted power was 6.6 kW and reached approximately 70% of the power for an optimized stable resonator. The divergence of the emitted laser beam in the unstable direction was lower than two times the diffraction limit. Experimentally measured margins for mirror misalignment were found in close agreement with numerical calculations.

A modified negative branch confocal unstable resonator (MNBUR) was coupled to the chemical oxygen-iodine laser (COIL) device of the German Aerospace Center. It consists of two spherical mirrors and a rectangular scraper for power extraction. Experimentally measured distributions of the near- and far-field intensities and the near-field phase were found in close agreement to numerical calculations. The extracted power came up to approximately 90% of the power as expected for a stable resonator coupled to the same volume of the active medium. The output power revealed a considerable insensitivity towards tilts of the resonator mirrors and the ideal arrangement of the scraper was found to be straightforward by monitoring the near-field distributions of intensity and phase. The beam quality achieved with the MNBUR of an extremely low magnification of only 1.04 was rather poor but nevertheless in accordance with theory. The demonstrated consistency between theory and experiment makes the MNBUR an attractive candidate for lasers that allow for higher magnification. In particular, it promises high brilliance in application to 100 kW class COIL devices, superior to the conventional negative branch confocal unstable resonator.

In an effort to reduce the potential risk of human exposure to chemical and biological hazardous materials, the demand increases for a detection system which rapidly identifies possible threats from a distance to avoid direct human contact to these materials. In this scope, we present a novel detection system which is able to measure simultaneously spectrally and temporally resolved laser induced fluorescence (LIF) signals excited by two consecutive laser pulses with different central wavelengths at 266 nm and 355 nm. As shown in this paper, the setup enables fast data acquisition that provides a complete dataset in less than a few milliseconds at repetition rates of 100 Hz. Furthermore, with its modular design it can be transported easily for operation at different locations. First measurements indicate a high performance with an accuracy of more than 97% in the distinguishability of bacterial specimen within a limited set of three representative bacterial species, namely Bacillus thuringiensis, Micrococcus luteus and Oligella urethralis. Together with the consecutive classification procedure, the setup promises to become a valuable tool for standoff detection of bio-hazards.

A new type of unstable resonator, suitable for a laser with a large medium cross section and a small or median output coupling, is presented. The resonator configuration, a modification of a negative-branch confocal unstable resonator, is numerically investigated. The basis of the theory is the Fresnel-Kirchhoff integral equation, and the calculations describe a passive resonator. With respect to output mirror tilting, the calculations confirm that the modified negative-branch confocal unstable resonator is less sensitive to mirror misalignments than the conventional negative-branch confocal unstable resonator. Furthermore, the modified resonator improves the beam quality in comparison with the conventional unstable resonator.