Biofilms are responsible for most of the interference caused by microorganisms in food processing. The aim of this study was to evaluate the cinnamon (Cinnamomum zeylanicum) essential oil and eugenol sanitizer and anti-biofilm activity against biofilms. Concentrations used of essential oil were 0.0% (control) 0.12%; 0.48%; 0.96% and 1.92%; the amount of eugenol was 0.76%. Concentrations were determined from other published studies. Number of viable cells and quantification the bacterial biomass were determined. Anti-biofilm treatment was effective in preventing the formation of biofilms. The 1.92% concentration was the most satisfactorily with Escherichia coli reduction of 5.91log CFUcm-2 and Staphylococcus aureus reduction of 5.17log CFUcm-2 (P<0.05) biomass of the two bacteria. Sanitizing treatment was not effective in reducing biomass. Seen this, the cinnamon and eugenol essential oil may be promising alternatives for controlling biofilms.

The objective of the study was to determine the antimicrobial activity antibiofilm and to identify the main components of the essential oil (EO) obtained from the leaves of Cymbopogon flexuosus. The antibacterial and antibiofilm activity was determined against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Salmonella Typhimurium ATCC 14028 and Listeria monocytogenes ATCC 19117. The effect of EO on biofilm was evaluated by quantifying viable cell number (CFU) and biomass by crystal violet (CV) analysis. The composition of the essential oils was determined by GC / FID and GC / MS. The results showed action against L. monocytogenes, S. aureus and S. Typhimurium with MIC and MBC values of 3.9 µL mL-1 , thus showing satisfactory antimicrobial activity, given this was the lowest concentration tested. For the antibiofilm activity, a significant reduction (P < 0.05) was observed for S. typhimurium and S. aureus. Biofilm biomass significantly reduced only for S. aureus and P. aeruginosa. EO presented the geranial and neral isomers as major components.

RESUMOO termo biofilme descreve a forma de vida microbiana séssil, caracterizada pela adesão de microrganismos a alguma superfície e com a produção de substâncias poliméricas extracelulares. Nas indústrias de alimentos, a formação de biofilmes resulta em graves problemas, já que pode ser fonte de contaminação do alimento, comprometendo a qualidade final do produto e a saúde do consumidor. O objetivo deste trabalho foi verificar a adesão de biofilmes (células sésseis) de bactérias patogênicas e/ou deteriorantes sobre superfícies da indústria alimentícia. As espécies bacterianas testadas foram Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, Listeria monocytogenes ATCC 19117 e Salmonella Typhimurium ATCC 14028. Utilizaram-se cupons de aço inoxidável e polipropileno como superfícies de contato. Os resultados demonstraram que P. aeruginosa e S. Typhimurium apresentaram maior capacidade de formação de biofilme. Estatisticamente, não houve diferença nas contagens das células de P. aeruginosa e S. Typhimurium (p > 0,05), o mesmo ocorreu entre L. monocytogenes e S. aureus. Entretanto, as contagens de células de P. aeruginosa e S. Typhimurium foram estatisticamente maiores que S. aureus e L. monocytogenes (p < 0,05). Através da microscopia eletrônica de varredura constatou-se também maior adesão de P. aeruginosa. Os resultados obtidos demonstraram que dentre as espécies bacterianas testadas P. aeruginosa foi a bactéria com maior capacidade de formação de biofilme.Palavras-chave: bactéria patogênica; adesão microbiana; aço inoxidável; polipropileno; qualidade do produto.

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