Patterns of highly glycosylated proteins with mainly cationic isoelectric points, pH 6.5 -9.5, were observed in serum and cerebrospinal fluid of patients with various disorders. Detection was performed after isoelectric focusing, using an immunoassay specific for digoxigenylated carbohydrate moieties of glycoconjugates. To our knowledge, these glycoproteins have not hitherto been described äs regulär serum proteins. The patterns were found among 7% of the patients studied (n = 400). Similar bands were not detectable in a reference group of 100 persons without clinical Symptoms. The glycoprotein pattern was specific for each individual. The pathophysiological meaning of these glycoproteins äs well äs the basic biochemistry has not yet been evaluated, The glycoproteins, however, were shown to differ from immunoglobulin G, oligoclonal immunoglobulin G, paraprotein or from regulär cationic serum protein. By comparison with Standard glycoproteins a carbohydrate content of 30 + 10% was roughly suggested. The oligosaccharides contain probably sialic acid äs evidenced by lectin binding. Although the diagnoses varied, 90% of the patients with this glycoprotein pattern had inflammatory processes.

Summary.After sham-feeding of glucose, conscious trained dogs bearing double-barrelled fistulas of the oeso-.phagus or of the stomach do not show any blood sugar mcrease. Nevertheless their Ii%I-lcvels in the peripheral venous blood increased considerably. This increase consists of two peaks of short duration between the 5th and the lOth as well as the 15th and the 25th min. Such IRI-peaks occured also after sham-feeding of tap water, but to a smaller extent. Their temporal order corresponds to the early IRI-peaks after oral glucose administration in intact animals, but before the blood glucose increase which was observed by us previously. After the application of glucose into the oral opening of the oesophageul fistula the first IRI-peak does not occur, the second and the third peaks appeared to the same extent. A feed-forward of insulin secretion induced by oral ingestion is suggested. It may be interpreted within the scope of the "entero-insular axis" of the mechanism of insulin secretion. Le mdeanisme de l'insulino-s~crdtion apr~s administration orale do glucose. IT. Libgration rdflexe do l'insuline chez le chien ~veillg portant des fistules du tractus digestif par alimentation simul~e de glucose ou d'eauRdsum& Des chiens dveillds et entralnds portant des fistules de l'oesophage ou de l'estomuc ne prdsentent aucune 61gvation du sucre sanguin apr@s ulimentation simul6e de glucose, ndanmoins nne augmentation consid@rable de la concentration insulinique duns le sang veineux pdriphdrique appuralt. Celle-ci consiste en deux pics brefs entre la 5 e et la 10 e minet aussi entre la 15 e et la 25e rain. Cos deux pics upparaissent aussi apr~s alimentation simulde d'eau potable, mais ~ un moindre degrd. Leur ordre chronologique correspond aux pics prdcoces d'IRI que nous uvons observds antgrieurement, apr6s administration orale de glucose chez des animaux intacts, avant l'616vation glyc6miquo rdelle. Apr6s application de glucose duns l'ouverture ahorale de la fistule chez des unimuux portant une fistule de l'oesophage, il manque le premier pic d'IRI, le deuxi~me et le troisi@me pic (provoquds par rdsorption) apparaissent invariablement. Line insulino-sdcrdtion intensifi~e, couplde ~ la prise de nourriture et induite par l'ingestion orale est suggdr@e et discutde duns le cadre de l'(~entero-insular axis~) du mgcanisme de l'insulino-s~cr6tion. Der Mechanismus der Insulinselcretion nach oraler Glucosegabe If. .Reflelctorische Insulinfreisetzung beim wachen Hund mit Fistetn dos Magen-Darm-Traktes durch Scheinfi~tterung mit Glucose odor Wasser.Zusammenfassung. Wache trainierte Hunde mit doppell/iuflgen Fistein des Oesophagus odor des Magens zeigen nach Scheinfiitterung mit Glucose keinen Bintzuckeranstieg, aber eine sichere Erh6hung des immunchemisch gemessenen Seruminsulins im peripheren Venenblut. Es wurden zwei kurzzeitige Peaks zwischen der 5. mad 10. sowie der 15. and 25. rain beobachtet. In geringerem Ausmal~ fanden sich diese beiden Peaks auch nach Scheinfiitterung mit Leitungswasser. Sic entsprechen in ihrer zeitlie...

Aus der II. medicinischen Klinik der Kgl. CharitY. Blutzerfall, Galle und Urobilin. Zur Frage der Gallenfarbstoffbildung aus Blur.

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