No abstract

Noninvasive Doppler ultrasonography (US) examination is a standard method for the clinical evaluation of the carotid arteries. Extracranial cerebral circulation includes the common carotid artery (CCA), the external carotid artery (ECA), and the extracranial part of the internal carotid artery (ICA). The present study was conducted with the objective of assessing physiological normative values and describing the appearance of spectral waveforms of extracranial arteries in 104 healthy dogs of eight breeds divided into four groups according to weight. We also focused on searching for correlations for carotid blood velocities with the resistive index (RI), body weight and diameter, and differences between observers and the influence of sex in the calculations of Doppler parameters. In the evaluated breeds, significant differences were found in the velocity of peak systolic velocity (PSV) and end diastolic velocity (EDV). There was a strong correlation between body weight and peak systolic velocity, the RI index and CCA diameter. The intra-observer agreement for the PSV and EDV parameters in each vessel was considered excellent reliability, and overall interobserver agreement showed very good reliability. This study could improve the descriptions of physiological values and waveforms recorded in carotid arteries. Defining the physiological values of velocity and the resistive index (RI) helps in the easier identification of pathology and diagnosis of disease. Our results may be used for further studies of vascular diseases in veterinary medicine that correlate with the pathology of neurological disorders of ischemic origin, further in thromboembolism, oncologic disease and degenerative, proliferative and inflammatory diseases of the arteries that lead to stenosis

This study was conducted to compare angles physically set on the stifle joints of cadaveric limbs of dogs with the results by four different radiographic methods for stifle angle measurement. Thirteen pelvic limbs from various large breeds and skeletally-mature dogs were used. The stifles were fixed at four angles: 125°, 130°, 135° and 140°. Altogether 52 radiographs were done. Each stifle angle set on the cadaver limbs was radiographically measured using four sets of landmarks (the goniometric, long axis, eminence and kinematic methods). We found similarity between angles physically set on cadaver limbs and radiographically measured using the long axis method (P > 0.05). The goniometric method showed similarity in group of limbs with the stifle fixed at 140° (P > 0.05), and other measurements differed significantly (P < 0.05). Eminence and kinematic method measurements were different compared to the angle of fixation of the stifle on the cadaver (P < 0.05) but similar compared to each other (P > 0.05). The method of stifle joint angle measurement should be considered when comparing similar studies, and also in pre-operative measurements for some tibial osteotomies aimed at stabilizing the joint after cranial cruciate ligament rupture.

Exosomes are nanovesicles that are involved in inter-cellular communication and are secreted by many types of cells. Exosomes secreted by stem cells can effectively transport bioactive proteins, messenger ribonucleic acids (mRNAs) and microribonucleic acids (miRNAs) organelles and play important roles in intercellular communication and the regulation of tissue regeneration. This transfer of bioactive molecules plays a main role in: tumor invasion and metastasis, immune and inflammation modulation, epithelial-mesenchymal transition and neurobiology. Mesenchymal Stem Cells (MSC) exosomes provide new perspectives for the development of an off-the-shelf and cell-free MSC therapy for the treatment of cartilage injuries and osteoarthritis. This report describes the progress in exosome studies and potential clinical use for osteoarthritis treatment.

This retrospective study was undertaken on the records of intraluminal diameter of the trachea in 185 dogs, in which hypoplasia of the trachea had been suspected. The relative size of the trachea was measured using the tracheal diameter (TD), thoracic inlet distance (TI), thoracic tracheal diameter (TT) and the width of the third rib (3R), expressed as ratios TD:TI and TT:3R. Thirty-five dogs were diagnosed as having tracheal hypoplasia. Bulldogs and non-bulldog brachycephalic dogs had significantly smaller measured trachea diameters compared to the predicted values calculated on the basis of their body weight. Radiographs of each dog were investigated by four observers. Inter- and intra-observer reliability (ICCinter, ICCintra) was based on the measurements taken by four observers to evaluate the reproducibility of the protocol. There was a good ICCinter (0.8) and ICCintra (0.89) agreement. Craniocaudal tangential radiographs, centred on the cranial thoracic aperture, did not show a significant difference in tracheal diameter measurements compared to the right lateral radiographs. In conclusion, our findings indicate that bulldogs and non-bulldog brachycephalic dogs have smaller tracheal diameters than non-brachycephalic dogs.