No abstract

Growing evidence suggests that metavirome changes could be associated increased risk for malignant cell transformation. Considering Viruses have been proposed as factors for prostate cancer induction. The objective of this study was to examine the composition of the plasma metavirome of patients with prostate cancer. Blood samples were obtained from 49 male patients with primary prostate adenocarcinoma. Thirty blood donors were included as a control group. The obtained next-generation sequencing data were analyzed using a bioinformatic pipeline for virus metagenomics. Viral reads with higher abundance were assembled in contigs and analyzed taxonomically. Viral agents of interest were also confirmed by qPCR. Anelloviruses and the Human Pegivirus-1 (HPgV-1) were the most abundant component of plasma metavirome. Clinically important viruses like hepatitis C virus (HCV), cytomegalovirus and human adenovirus type C were also identified. In comparison, the blood donor virome was exclusively composed of torque teno virus types (TTV) types. The performed HPgV-1 and HCV phylogeny revealed that these viruses belong to commonly detected in Brazil genotypes. Our study sheds light on the plasma viral abundance in patients with prostatic cancer. The obtained viral diversity allowed us to separate the patients and controls, probably suggesting that malignant processes may influence virome composition. More complex and multiple approach investigations are necessary to examine the likely causal relationship between metavirome and its nvolvement in prostate cancer.

Às minhas amigas da vida toda, Mirian Tainar, Aline Cardoso e Letícia Graça, que, mesmo de longe, vibram com as minhas conquistas e se fazem presente.Ao meu namorado, Jorge Woolcott, por ser minha calmaria e companhia nos dias difíceis. Obrigada por ter me dado forças para o intercâmbio, por me ouvir, aconselhar e ser meu melhor amigo.Ao meu irmão, Santiago Andrade, pois sem seu apoio e incentivo eu não teria chegado até aqui. Obrigada por acreditar nas minhas decisões. Agradeço também à minha irmã, Thayane Caetano por torcer pela minha felicidade e acreditar em mim.À minha mãe, Roseane Caetano, responsável pela pessoa que me tornei: determinada e forte. Obrigada por me ensinar sobre a vida, por toda educação e formação.Aos meus avós, Marleine Caetano e João Caetano, alicerces da minha vida. Serei eternamente grata por tudo.Palavras-chave: Câncer de próstata. Biomarcadores genéticos. Sangue periférico. Estratificação de risco. Jovens. Idosos.