Conceptualization and measurement of well-being have been carried out from different positions in positive psychology. In this cross-sectional study, the Scale of Positive and Negative Experiences (SPANE) has been adapted and validated on a sample of 458 Ukrainian adults. Confirmatory factor analysis has confirmed a two-factor model in which the positive affect (SPANE-P) and negative affect (SPANE-N) subscales were correlated (r = -0.594). The composite reliability was 0.909 for SPANE-P and 0.861 for SPINE-N. The mean removed variance was 0.630 for SPANE-P and 0.533 for SPANE-N, meaning converged validity is maintained. Discriminatory validity is satisfactory for both SPANE subscales, according to the Fornell and Larcker method and the HTMT test. The analysis of the measurement invariance of the SPANE testifies strict invariance for age and gender.