The temporal variability of planktonic ciliates was studied in an extreme oligotrophic environment with special focus on trophic modes and size classes. Abundance, biomass, size classes, mixotrophy vs. heterotrophy, and species composition of planktonic ciliates were investigated focusing on temporal (samples collected on a monthly basis during 2019) and vertical (7 depth layers in the euphotic zone, from surface to 120 m) distribution at a coastal station in the oligotrophic Eastern Mediterranean. Abundance was in general very low (20 to 1150 cells L–1), except for September, which presented the highest abundance and biomass. Aloricate species dominated the ciliate community in all months and depths (% contribution from 77% in September to 99% in April). In general, oligotrichs presented maximum abundance at 2–10 m (except for June, July, and November: 100–120 m) whereas choreotrichs were more homogeneously distributed [and showed maxima at deep chlorophyll maximum (DCM)]. Small heterotrophs dominated the ciliate community at all depths and months, on average by 76% (they were 3 times more abundant than mixotrophs in terms of abundance and 2.5 times in terms of biomass). They were equally distributed both vertically and seasonally (and also in terms of size classes). In contrast, mixotrophs were found mainly at the surface layer to 20 m throughout the year, except for June and July (max at 100, 50 m). On average, 63% of integrated aloricate abundance was species <30 μm, of which 25% were mixotrophs. During the stratification period of May to November, the very small (<18 μm) and small (18–30 μm) mixotrophic species were distributed throughout the water column whereas the >50 μm mixotrophic species were found only above the DCM. In contrast, during the mixing period of December to May, mixotrophic ciliates were very few and were dominated by small and medium-sized species. It seems that mixotrophic and heterotrophic ciliates, on one hand, and the four size classes, on the other, are very distinct groups characterized by different distributions both vertically and seasonally. This may have important consequences for the structure and function of the microbial food web of which they are part, as well as for the carbon flow to the higher trophic levels of this oligotrophic environment in which ciliates are the main grazers.

<p>In-situ oil burning (isOB) is one of the oil-spill mitigation measures and has been used after some major oil-spill events such as the Deepwater Horizon oil spill in the Gulf of Mexico. Although the ecological impact of oil spills and mitigation measures on the marine ecosystem are of great interest, the toxicity and biodegradation potential of isOB by-products have been poorly addressed and mainly are an unknown.</p><p>We investigated the effects of burned oil residue and soot deposition on the marine plankton communities of the oligotrophic Eastern Mediterranean Sea using a mesocosm experimental approach. Coastal water (collected at 300 m from the coast, north of Crete, Greece) was incubated in 3.5 m<sup>3</sup> mesocosms for 26 days. Three different treatments in triplicates were tested. In one treatment, 2 L of Iranian Crude Oil were added and burned (Burned treatment) while soot was collected and deposited in the form of artificial rain in more mesocosms (Soot treatment) using a custom-designed soot collection apparatus. The third treatment served as the non-contaminated control (Control treatment). Samples were collected at 9 time points (from Day 0 to Day 26) and the plankton, from viruses to micro-plankton, was studied using flow cytometry and inverted microscopy.  </p><p>Although the abundance of prokaryotes was slightly decreased in Burned and Soot treatments compared to the Control, the percentage of active heterotrophic bacteria and their relative size (based on their cytometric characteristics) was higher, which is an indication of increased bacterial activity in the contaminated treatments. Viral to Prokaryote Ratio and pico/nano-eukaryotic abundance were significantly increased in the Burned treatment compared to the Control, which could explain the lower bacterial abundance, despite the estimated increased activity, in the Burned treatment. Also, ciliate abundance was significantly lower in the Burned treatment while the opposite was observed in the Soot treatment (up to three orders of magnitude difference between Burned and Soot) compared to Control. Moreover, soot deposits seem to have had a positive effect on the abundance of dinoflagellates and diatoms.</p><p>To our knowledge, this is the first experiment to study the effects of isOB on whole marine plankton communities. It is evident that the in-situ oil burning has a significant effect on the plankton communities not only at the event site but also on distant areas through the soot deposition.     </p>

Ciliate conjugation is considered a rare event to encounter in the field and it is mostly reported from cultures. In this work, we describe a synchronized conjugation event of planktonic ciliates that was discovered twice; in September 2019, at two different locations in the Cretan Sea, Eastern Mediterranean, and in October 2020. In 2019, first, at 2 m depth of the coastal station POSEIDON-HCB, in samples fixed with acid Lugol and formaldehyde, we found 340 and 200 mating pairs L−1of different ciliate species, respectively; and second, at the Heraklion port, we found 220 mating pairs L−1 of Strombidinopsis sp. and 1960 mating pairs L−1 of Strombidium sp. At the Heraklion port visited again in 2020, we found 800 mating pairs L−1 of Strombidinopsis sp. and 200 mating pairs L−1 of Strombidium sp. Since detailed descriptions of conjugation in pelagic oligotrich ciliates are missing, our observations indicate that ciliate conjugation could be a frequent and periodic phenomenon, under specific conditions.