Abstrak Aktivitas fisik merupakan salah satu pilar pelaksanaan penanganan pasien diabetes mellitus. Pasien diabetes cenderung memiliki kekuatan massa otot dan fungsi tangan yang menurun yang dapat berakibat pada menurunnya kualitas hidup. Tujuan penelitian ini adalah mengetahui hubungan aktivitas fisik dan kekuatan massa otot terhadap kadar glukosa darah sewaktu pada pasien diabetes mellitus tipe 2. Penelitian ini merupakan penelitian deskriptif analitik dengan pendekatan observasional dan desain cross-sectional melibatkan 77 responden terdiagnosa diabetes mellitus tipe 2 berusia 18-60 tahun diwawancara mengenai aktivitas fisik menggunakan International Physical Activity Questionnare serta pengukuran kekuatan massa otot menggunakan hand dynamometer dan kadar gula darah sewaktu (GDS) menggunakan alat pengukur glukosa darah. Kadar GDS responden didominasi oleh kategori GDS tinggi yaitu 55 orang (71,4%), kategori kekuatan massa otot lemah memiliki persentase terbanyak yaitu 40 orang (51,9%), dan aktivitas fisik sedang sebanyak 46 orang (59,7%). Terdapat hubungan antara aktivitas fisik dan kekuatan massa otot dengan kadar gula darah sewaktu pasien diabetes mellitus tipe 2. Kata kunci: aktivitas fisik, kekuatan massa otot, gula darah sewaktu, diabetes mellitusAbstractPhysical activity is one of the most important interventions for patients with diabetes mellitus. Patients with this metabolic disease tend to have lower skeletal muscle strength that results in lower quality of life. This study aimed to examine the relation of physical activity and skeletal muscle strength on blood glucose level of type 2 diabetes mellitus patients. This study was an analytic observational study. The research subjects were 77 patients with type 2 diabetes mellitus aged 18-60 years old. Interviews were conducted on their physical activity habits using the International Physical Activity Questionnaire. Handgrip strength was measured using hand dynamometer, while the rapid blood glucose was measured using a blood glucose monitoring device. Most prevalent blood glucose level category in this study was “high” with 71.4% of respondents, 51.9% of respondents had low skeletal muscle strength, and 59.7% of respondents had moderate physical activity level. There is a significant relationship between physical activity and skeletal muscle strength of patients with type 2 diabetes mellitus blood glucose levels. Keywords: physical activity, skeletal muscle strength, blood glucose level, diabetes mellitus