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The Multicenter International Project suggests Zincum Mettalicum high diluted as object of study in different experimental models. Aim: evaluate the effect of substance high diluted Zincum metallicum in murine experimental infection by Trypanosoma cruzi. Metodology: was performed a blind, controlled, randomized, using 60 swiss male mice, 56 days old, divided into groups: CNI - uninfected and untreated animals; CI - infected and untreated animals; infected and treated animals: ZN5cHTA - Zinc 5ch and LAC5cHTA - Lactose 5ch , 48 hours before and after infection, subsequently were treated 56/56 hours until 9th day of infection; ZN5cHTTD - Zinc 5Ch and LAC5cHTTD - Lactose 5cH, everyday from the 4th of infection. Animals were inoculated with 1.400 blood trypomastigotes, strain Y-T. cruzi, intraperitoneally. Medicines were handled, prepared in grain alcohol 70%, and dynamized up to 100 times until 4cH. To obtain the 5cH it was used bi-distilled sterilized water filtered in membrane - 0.22 µm [1], on separate days (first Lactose and then Zinc) and stored in different rooms. Microbiological test in vivo and toxicity were made in accordance with current legislation [2].Test solutions were diluted in water at a concentration of 10% (1mL/100mL) after dilution in water. Clinical (temperature, weight, water/foodintake and excreta)[3] and parasitological parameters (pre-patent and patent period, peak parasitemia, and parasitemia overall survival time)[4] were assessed daily. Data were compared BioEstat 5.0, significance level of 5%. Project was approved by the Ethics Committee on Animal Use in Experimentation of the Universidade Estadual de Maringa by opinion number 025/2014. Results: ZN5cHTA group had a higher survival rate than their control LAC5cHTA (p=0.004). ZN5cHTA shows 55.7% probability of surviving to the 15th day after infection, while LAC5cHTA 29.4%. ZN5cHTA also provides significantly better performance (p= 0.0206) compared to CI, contrary to what occurs with LAC5cHTA x CI (p=0.7410). There is no significant difference in survival between the different treatments schemes TA and TTD, either with ZN5cH (p=0.0754) or LAC5cHTA (p=0.9480), although the best ZN5cHTA present trend toward benefit. Considering parasitological parameters ZN5cHTA group had higher pre-patent period (PPP) meaning benefit to infected animals [5]. Although ZN5cHTA shows greater number of parasites from 6th to 11th day of infection and right shift of parasitemia peak in relation to LAC5cHTA (p=0.020), this group displayed a better performance compared to the other groups as observed in other models [6]. Conclusion: ZN5cHTA group had higher survival, greater pre-patent period and better clinical outcome compared to control LAC5cHTA and to other groups. This result may be related to higher total parasitemia and alterations in the parasite cycle time observed in this group. These findings suggest aggravation with posterior benefit as reported in some cases of homeopathic treatment.

The objectives of this prospective, experimental study were to describe changes in the stiffness of the equine superficial digital flexor tendon (SDFT) after induced injury, deep digital flexor tendon (DDFT), accessory ligament (AL‐DDFT), and suspensory ligament (SL) during 90 days of healing using acoustic radiation force impulse (ARFI) elastography. Eight healthy horses were selected. Preinjury B mode and ARFI evaluations were performed bilaterally in the palmar metacarpal region. Injury was induced only on the left forelimb (G2) by a single injection of collagenase in SDFT, 15 cm distal to the accessory carpal bone. The right forelimb was used as a control (G1). Evaluations were performed at eight timepoints: one before injury (T0) and seven (T1‐T7) after injury (3, 15, 30, 40, 60, 75, and 90 days post‐induction). Tendinopathies were visualized as hypoechoic areas with loss of parallel tendon fiber pattern. Injured SDFTs presented mainly cool colors (soft) from T1 to T3, and from T4, there was an increase in warm colors (hard), close to the appearance of tendons of G1. In the first four timepoints, there was a decrease in stiffness compared to G1 (P < 0.001). On T1 and T2, a cutoff value <6.21 m/s to determine tendinopathy of the SDFT was established (75.8% sensitivity and 92.03% specificity). Stiffness changes in the DDFT, AL‐DDFT, and SL of injured limbs occurred at different timepoints. Tendinopathy significantly altered the stiffness of the injured tendon and the adjacent tissues. ARFI made it possible to detect these changes, helping to monitor the reparation of this injury.

Although several diseases are treated by toxic drugs, their side effects may hamper adherence to the therapy. The aim of this study is to evaluate the effect of the association of ponderal benznidazole (BZ) with its ultra-high diluted (UHD) formula on clinical and parasitological parameters of mice infected by Trypanosoma cruzi (T. cruzi). 24 non-isogenic Swiss mice were divided into groups: CI – infected animals treated with 7% alcohol; BZp – infected animals treated with BZ (500 mg/ kg) from the beginning of infection; BZp+d – infected animals treated with ponderal BZ and with UHD BZ, which started to be administered four days after the beginning of treatment with ponderal BZ; CNI - group of non-treated and non-infected animals. The UHD medicine was prepared according to Phamacopoeia until 30x. The different treatment schedules were statistically compared through parasitological and clinical parameters. The group BZp+d displayed more favorable clinical evolution than the group BZp, with improvement of mass gain, feed conversion and water intake, presenting data approximated to CNI group. The significant increase of the body temperature of BZp+d group indicates an activation of the immune system which was not observed in the other groups. Moreover, the anti-parasitic effect of the ponderal drug was maintained in all parasitological parameters of this group. By reducing the side effects and maintaining the action of the ponderal drug, the combination of toxic drugs with their UHD formula could be considered a way of improving efficacy of the treatment.