No abstract

Following recent work on new Tris hydroxymethyl aminomethane Schiff base derivatives were synthesized and characterized by using NMR ( 1 H, 13 C, and depth), FT-IR, and Mass spectroscopy. The crystal structure of STB has been determined by X-ray diffraction analysis. The binding interaction of the 3 chemically synthesized molecules with human serum albumin has been examined under the pH = 7.40 through UV-visible absorption and fluorescence spectroscopy analysis. The result obtained from the fluorescence experiment (10 14 ) suggests a static mechanism of quenching. By utilizing fluorescence spectroscopy to determine the binding constant (K b = 10 6 ), it was determined which ligands have the highest affinity for HSA and that these ligands had changed the structure of HSA. Through hydrophobic interactions, the ligands bind to HSA on site I (subdomain II), according to thermodynamic parameters like enthalpy change (ΔH o ), entropy change (ΔS o ), and Gibbs free energy change (ΔG o ). The result of 3D fluorescence spectra also showed that albumin conformational changes were brought on by these ligands. The results of the experiments were supported by DFT and molecular docking of ligands with HSA. Escherichia coli, Stap. aureus, Aspergillus niger, and Aspergillus flavus were tested for antimicrobial activity against the synthesized compounds respectively.