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The usefulness of RT-PCR for the detection of MHV in tissues and feces of experimentally infected animals has been reported, but it was unclear whether the method was also applicable for the detection of MHV during a natural outbreak. Enterotropic infection is considered to be the most common form of natural infection among various forms of MHV infection. In this paper, RT-nested PCR was performed to detect MHV excreted in the feces during an outbreak in an immunocompromised A/WySnJ mouse colony. The expected bands were amplified after nested PCR from 20 fecal samples out of 37. These results showed that RT-nested PCR could be applicable for the diagnosis for MHV natural infection.

Localizationof Pasteurella pneumotro pica was investigated in the respiratory tract, conjunctiva and vagina of 5-week-old, 10-week-old and retired asymptomatic mice and rats.The highest isolation rate of the organisms was obtained in the pharyngolarynx, showing 85 to 97.5% in carrier mice and 100% in carrier rats. Numbers of the organisms in this site were 103-5 and 107-8 organisms/g tissue in 4-week-old mice and rats, respectively. Isolation rates in the nasal cavity and trachea of the both animals were not so high as those in the pharyngolarynx, but usually higher than those in the external nares.The organisms were rarely isolated from the lung.Isolation of the organisms from the conjunctiva was common in rats, especially in young ones, but rare in mice. About 30% of carrier mice and 50 to 100% of carrier rats harbored the organisms in their vaginas.

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Bacteriological and serological examinations were made on Pasteurella multocida infection in two rabbit-breeding colonies.The organism was localized in the paranasal sinuses of 53 of 54 infected rabbits, excreting to the external nares of 49 rabbits.It was also isolated from the trachea and middle and inner ear of half of the infected animals and occasionally from the conjunctiva, lung and heart.The infection rate of the organism was very low (4.30) in sucklings younger than a month old, but increased with advancing age, reaching nearly 100% in adults more than five months of age. A rapid increase of the infection rate was observed at two to three months of age. Serum antibody against somatic antigen of P. multocida was demonstrated in infected rabbits by use of the tube agglutination test, showing a close correlation with isolation of the organism in adults. Sucklings younger than one month of age from infected dams were significantly resistant to experimental nasal infection of P. multocida.