Smad3 deficiency attenuated renal fibrosis, inflammation, and apoptosis after UUO, suggesting that Smad3 was a key molecule mediating TGF-beta activity leading to real fibrosis after UUO.

No abstract

For mirror nuclei with masses A=42-95, the effects of isospin-nonconserving nuclear forces are studied with the nuclear shell model using the Coulomb displacement energy and triplet displacement energy as probes. It is shown that the characteristic behavior of the displacement energies can be well reproduced if the isovector and isotensor nuclear interactions with J=0 and T=1 are introduced into the f(7/2) shell. These forces, with their strengths being found consistent with the nucleon-nucleon scattering data, tend to modify nuclear binding energies near the N=Z line. At present, no evidence is found that these forces are needed for the upper fp shell. Theoretical one- and two-proton separation energies are predicted accordingly, and locations of the proton drip line are thereby suggested.

The identification of human CD34-negative (CD34−) hematopoietic stem cells (HSCs) provides a new concept for the hierarchy in the human HSC compartment. Previous studies demonstrated that CD34− severe combined immunodeficiency (SCID)-repopulating cells (SRCs) are a distinct class of primitive HSCs in comparison to the well-characterized CD34+CD38− SRCs. However, the purification level of rare CD34− SRCs in 18 lineage marker-negative (Lin−) CD34− cells (1/1000) is still very low compared with that of CD34+CD38− SRCs (1/40). As in the mouse, it will be necessary to identify useful positive markers for a high degree of purification of rare human CD34− SRCs. Using 18Lin−CD34− cells, we analyzed the expression of candidate positive markers by flow cytometric analysis. We finally identified CD133 as a reliable positive marker of human CB-derived CD34− SRCs and succeeded in highly purifying primitive human CD34− HSCs. The limiting dilution analysis demonstrated that the incidence of CD34− SRCs in 18Lin−CD34−CD133+ cells was 1/142, which is the highest level of purification of these unique CD34− HSCs to date. Furthermore, CD133 expression clearly segregated the SRC activities of 18Lin−CD34− cells, as well as 18Lin−CD34+ cells, in their positive fractions, indicating its functional significance as a common cell surface maker to isolate effectively both CD34+ and CD34− SRCs.

No abstract