We demonstrated a high-power
Q
-switched two-stage Ho:YAG master-oscillator power-amplifier (MOPA) system dual-end pumped by Tm:YLF lasers. A new method was introduced by rotating and swapping spatial axial directions of pump beams to improve the beam quality of the Ho:YAG oscillator and first-stage amplifier. Two parallel second-stage Ho:YAG amplifiers were employed to output high power. A total maximum average output power of 332 W at 2091 nm with pulse repetition frequency of 20 kHz was achieved. Then a
Z
n
G
e
P
2
MOPA system was demonstrated using the Ho:YAG MOPA as the pump source. A maximum average output power of 161 W at 3–5 µm was obtained with 290 W incident Ho pump power, corresponding to beam quality factors
M
2
of 3.42 and 3.83 for horizontal and vertical directions, respectively.
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