The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.
Although disorders of the stomatognathic system are common, the mechanisms involved are unknown. Our objective was to study the changes in the masseter muscles after unilateral exodontia. Molar extraction was performed on Wistar
rats (left side), and the animals were sacrificed after either 14 or 26 days. The masseter muscle was processed for histological analysis, conventional and in situ zymography, and immunohistochemistry. The morphological analysis showed unique and specific characteristics for the experimental group. By conventional zymography no significant values of 72 kDa MMP-2 (P < 0.05) were found in both of the sides of masseter muscle after 14 and 26 days of unilateral extraction. The in situ zymography showed gelatinolytic activity on all deep masseter muscles, with significant increase on the contralateral side after 14 and 26 days (P < 0.05). The immunohistochemistry demonstrated greater expression of MMP-2 than MMP-9 and MMP-14 in all masseter muscles and there were few differences in the staining of 4 TIMPs. This knowledge about morphology and molecular masticatory muscle remodeling following environmental interventions can be used to develop clinically successful treatments.
O objetivo deste estudo foi avaliar a estabilidade da cor do esmalte dentário exposto à fumaça de cigarro após a descolagem ortodôntica. Métodos: Trinta e dois incisivos bovinos foram alocados nos grupos controle (C 1 and C 2 ) e experimental (n = 8) de acordo com protocolos de colagem ortodôntica distintos: com adesivo (B 1 ) e sem adesivo (B 2 ) e expostos à fumaça de cigarro. Amostras do B 1 , B 2 e C 2 foram expostas a dez ciclos de fumaça em uma câmara específica e hermética, enquanto o C 1 permaneceu armazenado em saliva artificial. A análise da estabilidade de cor foi realizada com um espectrofotômetro de acordo com o sistema L* a* b*. As comparações intergrupos e o efeito do tempo foram verificados com ANOVA / Tukey e testes t de Student, respectivamente (a=0,05). Resultados: Não foram observadas alterações de cor estatisticamente significativas no C 1
INTRODUÇÃO: A má oclusão de Classe III pode ser tratada ortodonticamente na fase da dentição mista. Contudo, outros tipos de más oclusões associadas, como a sobremordida exagerada, podem agravar a severidade do caso. Diante disso, a necessidade da utilização da máscara facial fica comprometida pela ausência de uma dimensão vertical livre suficiente que compense esse trespasse vertical acentuado, dificultando, dessa forma, a protração da maxila e consequente descruzamento dentário anterior. Como solução, torna-se bastante comum a confecção e cimentação de batentes de desoclusão posterior fixos. Mas e quando não há suporte dentário posterior satisfatório para permitir a instalação desses batentes? O que fazer? OBJETIVO: O objetivo do presente artigo foi descrever como obter desoclusão posterior para o tracionamento maxilar na fase da dentição mista, nos casos com sobremordida exagerada associada e poucos elementos dentários na região posterior.
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