Context:Stachys guyoniana Noë ex. Batt. and Mentha aquatica L. are two Algerian Lamiaceae used in folk medicine.Objective: To investigate their antioxidant, anticholinesterase and antibacterial activities.Material and methods:n-Butanol (BESG), ethyl acetate (EESG) and chloroform (CESG) extracts of S. guyoniana and methanol (MEMA) and chloroform (CEMA) aerial part extracts of M. aquatica and methanol (MERMA) and acetone (AERMA) roots extracts of M. aquatica were evaluated for their antioxidant activity by the β-carotene-linoleic acid, DPPH• and ABTS•+ scavenging, CUPRAC and metal chelating assays. The anticholinesterase activity was tested against AChE and BChE. The antibacterial activity was assessed by MICs determination against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella heidelberg, Klebsiella pneumoniae, Enterobacter aerogenes and Morganella morganii strains.Results: In the β-carotene test, the CESG (IC50: 2.3 ± 1.27 μg/mL) exhibited the highest activity. The BESG was the best scavenger of DPPH• (IC50: 2.91 ± 0.14 μg/mL). In the ABTS test, AERMA was the most active (IC50: 4.21 ± 0.28 μg/mL). However, with the CUPRAC, the BESG exhibited the best activity (A0.50: 0.15 ± 0.05 μg/mL) and was active in metal chelating assay with 48% inhibition at 100 μg/mL. The BESG was the best AChE inhibitor (IC50: 5.78 ± 0.01 μg/mL) however, the AERMA showed the highest BChE inhibitory activity (IC50: 19.23 ± 1.42 μg/mL). The tested extracts exhibited a good antibacterial activity.Conclusion: This study demonstrated good antioxidant, anticholinesterase and antibacterial potential of S. guyoniana and M. aquatica, which fits in well with their use in folk medicine.

From ages, dates are consumed by humans for its beneficial health and nutritional values. The aim of this study was to evaluate antiinflammatory effect of Algerian date fruit (Phoenix dactylifera). A quantity of 50 mg/kg of the P. dactylifera extract was analyzed in vivo using the formalin-induced paw edema test in mice. The inflammation level was detected by the measure of the edema size, also the dosage of C-reactive protein (CRP) and homocysteine levels in the blood. The results showed a highly significant decrease in the edema size and the level of the homocysteine in the blood (P = .000) and significant decrease in CRP values (P < 0.05) in the 50 mg/kg plant extract group compared to Control, Methionine 400 mg/kg and (Plant + Methionine) groups. The study suggests that the extract possesses enough potential to reduce inflammation by in vivo and directs the importance of further research and development of novel anti-inflammatory agents. ARTICLE HISTORY

A pentacyclic triterpene, named salvibuchanic acid (1), together with five known compounds, were isolated from the roots of Salvia buchananii Hedge (Lamiaceae). The structural characterisation of all compounds was performed by spectroscopic analyses, including 1D and 2D NMR and HRESIMS experiments. The lupane triterpene (1) and hyptadienic acid (2) were investigated for their potential cytotoxic activity on Jurkat, HeLa and MCF7 cell lines. Both compounds showed an interesting antiproliferative activity with similar potency in all cell lines. By means of flow cytometric studies, hyptadienic acid (2) induced in HeLa cells a S cell cycle block, while 1 elicited both cytostatic and cytotoxic responses.

Four new phenylethanoid glycosides (1: -4: ), 1--hydroxyphenyl-ethyl-2--acetyl-3,6-di--l-rhamnopyranosyl--d-glucopyranoside (1: ), 1---hydroxyphenyl-ethyl-3,6--di--l-rhamnopyranosyl--d-glucopyranoside (2: ), 1---hydroxyphenyl-ethyl-2--acetyl-3,6-di--l-rhamnopyranosyl-4--coumaroyl--d-glucopyranoside (3: ), and 1---hydroxyphenyl-ethyl-3,6-di--l-rhamnopyranosyl-4--coumaroyl--d-glucopyranoside (4: ), together with three known compounds, were isolated from the -butanol extract of aerial parts. The structural characterization of all compounds was performed by spectroscopic analyses, including 1D and 2D NMR, and HRESIMS experiments. The isolated compounds were assayed for their inhibitory activity on two enzymes involved in the peculiar glycolytic or lipidic metabolism of cancer cells, human lactate dehydrogenase (LDH), and monoacylglycerol lipase (MAGL), respectively. All the compounds showed negligible activity on LDH, whereas some of them displayed a certain inhibition activity on MAGL. In particular, compound 1: was the most active on MAGL, showing an IC value of 88.0 µM, and modeling studies rationalized the supposed binding mode of 1: in the MAGL active site.

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