Synthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and cost-effective technique. Trillium govanianum Wall. Ex. Royle crude extract was used for the eco-friendly genesis of silver nanoparticles (AgNPs). Aromatic amines were the functional groups involved in the bio-fabrication and synthesis of the AgNPs. The production of AgNPs was established by the appearance of brown color. The manufactured AgNPs were characterized by UV-Vis spectrophotometer, X-ray diffractometer, and FTIR spectrophotometer. AgNPs were face-centered cubic in nature with an average size of 9.99 nm. The produced AgNPs (18 µL disc−1) showed substantial antibacterial (53.74, 52.75, 51.61, 43.00, 36.84, and 36.84%) and antifungal (54.05, 42.11, 41.10, 40.85, 30.55, and 29.73%) potential against the tested bacterial (X. campestris, P. aeruginosa, S. aureus, E. coli, B. subtilis, and K. pneumoniae) and fungal (A. alternaria, Paecilomyces, C. albicans, Curvularia, A. niger, and Rhizopus) strains, respectively.