Naringin is a flavonoid which shows various pharmacological effects, such as, anti-inflammatory and antioxidant,cholesterol lowering activity, free radical scavenging activity. Although naringin is easily found in citrus fruits but has lower bioavailability, biodistribution and undergoes biotransformation to naringenin. To overcome this, the main objective of this work is to formulate  nanoethosome formulation containing naringin.  The use of nanoethosomes as vesicle drug carrier having ability to increase solubility, improve biodistribution, slows the biotransformation which improves the activity of naringin for treating neurological disorder. The ethosomes were formulated by varying the variables such as concentrations of soya lecithine, polyethylene glycol, and ethanol. The formulations were evaluated  with entrapment efficiency, and particle size. Results specify that prepared nanoethosomes of naringin shows decreased particle size, better entrapment efficiency as compared to rigid ethosomes. The F4 was selected as optimized formulation which was further characterized for vesicle size determination. The F4  shows vesicles size of 145.9 nm having 83.9% entrapment efficiency. The nanoethosomes were  proved to be significantly superior in terms of amount of drug permeated into the skin, with an enhancement ratio of 3.77 when compared to rigid ethosomes. Our results suggests that nanoethosomes are an efficient carrier for improved naringin permeation & stability.

The aim of the present research work is to design miconazole-loaded chitosan nanoparticles that could potentially assemble in wrinkle and hair follicles to provide prolong release to the skin tissue. The amount of drugs required for the preparation of nanoparticles was determined by studying the entrapment efficiency of preliminary batches. The emulsification/Solvent evaporation method was used for the preparation of nanoparticles. Different proportions of Miconazole Nitrate and Chitosan were dissolved in DCM. The size of the globules in the emulsion was reduced by a high energy shearing using a probe Sonicator at 50 % amplitude for 10 Minutes, followed by the addition of 10 ml 2% PVA. After overnight evaporation of DCM, for isolation of dried NPs, the NPs dispersion was centrifuged at 15,000 RCF for 30 minutes. The obtained particles were dispersed in de-ionized water and freeze-dried. 32 full factorial design was selected for optimization purposes. Prepared batches were evaluated for various parameters such as entrapment efficiency, production yield, particle size, zeta potential, and SEM. F5 batch was found to be optimized which was then used for the preparation of gel. Three levels of Carbopol934 and propylene glycol were used for the optimization of gel. The prepared gel was also evaluated for pH, drug content, viscosity, and spreadability. From the study, it was concluded that nanoparticle gel can be used for the treatment of various skin infections over the conventional gel.

Ipomoea obscura (L.), commonly described to this in Ayurveda as "Laksmana, " is an herb in the Convolvulaceae family. It's a little climber with small cordate leaves with apex that's acuminate. Five fully united petals make up the corolla. In disturbed environments, the plant grows on fences or as a low ground cover as a substrate. It's a slender, twinning perennial herb that grows in grasslands, hedges, and wastelands all over India, up to a height of 3000 feet. As a climber with lovely flowers, it also has decorative value. [1] This plant's medicinal benefits have been found by Ayurveda, and it is used to treat diarrhoea, open sores, and pustules. Ulcers, haemorrhoids, and swellings are treated with a leaf paste. Fruits and seeds are being used as purifying agents, to help with difficult breathing, to relieve pain, and also to improve vision. [2] To treat colds, asthma, and dry cough, gingely oil is combined with the fresh plant extract.By widening blood vessels, the plant extract relieves pain in the body. Many siddha medications contain plant extracts. [3] Other phytochemicals found in this plant were flavonoids, steroids, essential oil, and phenolic acids. [4] This plant contains indole alkaloids like Ipobscurine A, Ipobscurine B, Ipobscurine C, and Ipobscurine D as well as tropane alkaloid including Calysteginine B-1,2, 3 and 4. Calysteginine C-1; [5][6] Ipobscurine-A, C, and D are significant pharmacologically active macrolactum type indole alkaloids found in Ipomoea obscura.

In the present work new, Simple Reversed-Phase High Performance Liquid Chromatographic method was developed and validated for the estimation of Melatonin in tablets. A sharp peak was obtained 7.125 min using Methanol: Phosphate buffer (PH 3.0) in the ratio of 40:60 as mobile phase at a flow rate of 1.0 ml/min, on HPLC Waters 515 system containing UV detector with Empower software. The separation was achieved on the C18 analytical column (250 mm × 4.6 mm, 5.0 μm). The detection was carried out at 223 nm. The method gave good resolution and suitable retention time. The results of the analysis in this method were validated in terms of specificity, accuracy, precision, linearity, and range. The method was found to be reliable, reproducible, rapid, and economic. The RP-HPLC method developed in this study showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for the estimation of Melatonin in tablets.

Aim: The present work was aimed to develop and evaluate polyherbal-based novel antiaging formulation. Place and Duration of Study: Faculty of pharmacy, B N University Udaipur, Department of Pharmacognosy, Divine College of pharmacy Satana between Feb 2019 to Apr 2021. Methods: The selected plant extract Moringa oleifera hydroalcoholic extract (2%), Juglans regia aqueous extract (1%), Vitis vinifera ethanolic extract (1.5%), Camellia sinensis cold water extract (1.8%), Punica granatum aqueous extract (2%) were optimized in o/w type herbal cream by incorporation of different concentration of stearic acid and Tween 60. The total 9 formulations were evaluated on the basis of preliminary, phytochemical screening, and accelerated stability study and confirm the C7 polyherbal formulation (PHF) were more stable, safe, and showed pseudo plastic flow. Results: The In-vitro free radical scavenging assay (DPPH Assay - IC50 56 ± 0.04 µg/ml and H2O2 scavenging assay - IC50 = 67±0.68 µg/ml) compared with standard Ascorbic acid and In-vitro anti-collagenase and anti-elastase activity validate PHF as antioxidant and antiaging activity. The in vitro Anti-collagenase activity showed 89.5% inhibition at 100 μg/mL concentration of C7 formulation (IC50 = 54.62 μg/mL) and the standard Gallic acid showed 74.6% inhibition at 100 μg/mL (IC50 = 67.83 μg/mL). The in vitro Anti-elastase activity showed 67.5% inhibition at 250 μg/mL of C7 formulation (IC50 = 193.65 μg/mL) and copper sulphate solution used as standard showed 70.6% inhibition (IC50 = 772.42 μg/mL). The percent inhibition activity was observed that the C7 formulation is potential antiaging activity as compared to positive control. Conclusion: These studies conclude that the composition of PHF with a cream base increases the production of collagen and elastin which is responsible for the noteworthy synergistic antiaging activity.