Biochemical parameters are greatly influenced by genetic, physiological and environmental factors. Regional data is essential to establish a relationship between End stage renal disease (ESRD) and biochemical parameters. In Bangladesh, there is not any established research data on biochemical parameters of ESRD patient. The present study was a case control study with 500 ESRD patients and equal number of healthy volunteers (controls). Sociodemographic, anthropometric and clinical data of both patients and controls were collected. Serum biochemical parameters were analyzed by laboratory test. Statistical software package SPSS were used for independent sample t-test and Pearson's correlation test. Our study found that serum creatinine, blood urea nitrogen (BUN) and serum uric acid were significantly higher (p<0.05) and serum potassium were significantly lower in ESRD patient as compared to control subjects (p<0.05). But serum chloride, serum sodium and serum calcium level were in the normal range. Pearson correlation analysis reveals that serum creatinine and serum chloride was inversely correlated with GFR (glomerular filtration rate) in both patient and control groups. This study explored that ESRD patients have higher level of serum creatinine, BUN and serum uric acid but lower level of serum potassium than normal patient. Routine investigation of serum biochemical parameters may help to prevent ESRD complication.