The aim of the study was to assess the effects of training on haematological and biochemical blood parameters as well as on the changes in body surface temperature in horses. In order to identify the predictive value of surface temperature measurements as a marker of animal's performance, their correlations with blood parameters were investigated. The study was carried out on nine horses divided into two groups: routinely ridden and never ridden. Infrared thermography was used to assess surface temperature changes before (BT) and just after training (JAT) on a treadmill. Seven regions of interest (ROIs) located on the neck, shoulder, elbow, back, chest, gluteus and quarter were analysed. The blood samples were taken BT, JAT and 30 min after training (30AT). Haematological parameters including white blood cells, lymphocytes (LYMs), monocytes (MONOs), granulocytes (GRAs), eosinophils (EOSs), haematocrit (HCT) and platelets (PLTs) as well as biochemical parameters such as glucose (GLUC), urea, chemnormalNa+, chemnormalK+ and chemnormalCa2+, and creatine phosphokinase (CPK) were analysed. Our results indicated a significant increase in surface temperature JAT (p=0.043) in the neck, shoulder, elbow, gluteus and quarter in routinely ridden horses. Significant changes in EOS (p=0.046) and HCT (p=0.043) in the case of the never-ridden and routinely ridden group, respectively, were found between the times of blood collection. In addition, there was a significant effect of the horse group and the time of blood collection on the CPK activity (p=0.025 to p=0.045) and urea concentrations (p=0.027 to p=0.045). In the routinely ridden horses, there were significant correlations between the changes in MONO (ρ=0.40), GRA (ρ=-0.40), PLT (ρ=-0.77), HCT (ρ=-0.36), GLUC (ρ=0.56) and urea (ρ=0.56) and the total ROI temperature changes. Moreover, significant correlations between the changes in MONO (ρ=-0.86), EOS (ρ=-0.65), GLUC (ρ=0.85), urea (ρ=0.85), chemnormalNa+ (ρ=0.59) and chemnormalK+ (ρ=-0.85) and the total ROI temperature changes were found in never-ridden horses. Different changes in body surface temperature and blood parameters in routinely ridden and never-ridden horses could be associated with different conditioning and performance. A significantly higher surface temperature in routinely ridden horses, as well as the dynamics of changes in HCT, CPK and urea after training indicate better performance of these horses. Significant correlations between MONO, GLUC, and urea and a total ROI surface temperature as well as a negative correlation between MONO and the total ROI temperature in never-ridden horses indicated poor performance.

This study aimed to estimate the influences of flax oil and a mixture of ethyl esters of fatty acids from flax oil added to a milk replacer (MR) formulation on the biochemical, immunological, and antioxidant status of blood, production parameters, and health of calves. Twenty-seven Holstein-Frisian calves were assigned to 3 groups: the control group without fat added to the diet (CON, n = 9), a group receiving ethyl esters of flax oil (10 g/d) with lyophilized apples (25 g; MRE), or a group receiving flax oil (10 g/d) with lyophilized apples (25 g; MRL). The study was conducted from d 14 to d 42 of life. Intake of MR and feed were recorded daily, and fecal scores, rectal temperature, and body weight were recorded weekly. Laboratory blood tests were conducted every 7 d from d 14 to 42. Supplementation with the formulation containing ethyl esters of flax oil (MRE) positively affected health, average daily weight gain, growth rate, and feed efficiency. Intake of the starter feed was lower in the calves receiving the formulation with flax oil (MRL). The MRE formulations lowered the concentrations of triglycerides, total cholesterol, and LDL fraction in blood serum and decreased the total antioxidant capacity (TAS) and levels of glutathione peroxidase (GPx). The MRL formulation increased TAS and GPx levels in blood serum. The MRE treatment limited the circulating levels of tumor necrosis factor. The MRE and MRL preparations lowered the concentration of SFA (C16:0, C18:0, C18:1) in blood serum, and MRE significantly increased levels of α-linolenic acid. The MRE and MRL preparations beneficially affected production and physiological parameters. Our data support the idea that supplementation with n-3 fatty acids improves calf growth and metabolic and oxidative functions in young calves.

Vegetable and animal oils are susceptible to the oxidation of their lipid components on storage. Polyphenols from apple peels are investigated as potential natural antioxidants used for stabilizing polyunsaturated fatty acid and preventing oxidation. The aim of this study was assessing the antioxidant efficacy of apple pomace as natural antioxidant in linseed and fish oils, stored in anaerobic conditions. Apple pomace was added to the linseed and fish oils stored for eight weeks to evaluate the antioxidant activity of their polyphenolic components. The total phenolic content, activity of DPPH, ABTS•+, FRAP, acid value (AV), peroxide value (PV), and fatty acid profile were analyzed in storage tests. We found that apple pomace, regardless of the oil content of the formulation, was capable of blanking 2,2-diphenyl-1-picrylhydrazyl radicals. The highest ability to reduce Fe3+ ions occurred in the samples containing 30% of the fish oil. The use of apple pomace comprising polyphenolic compounds improves the stability of linseed and fish oils in storage tests. Polyphenols in apple pomace show a high antioxidant potential, as indicated by their values of DPPH, ABST•+, and FRAP. The addition of apple pomace resulted in limiting the acid and peroxide values of the samples during storage.

Simple SummaryThe beneficial effect of herbs on production parameters, quality of products of animal origin, and animal health was demonstrated in numerous studies. This study aimed to determine an effect of herbal mixture on blood parameters and the anti-colibacteriosis efficacy. The rabbit model used during the experimental infection with E. coli indicates a clear effect of Rumex crispus L., Pontentilla anserine, Polygonum aviculare, whose activity involves the reduction of colonization of basically all sections of the intestines. The use of herbs in rabbits can control the activity of intestinal microbial community. Administration of a mixture of herbs to feed reduced the number of E. coli in cecum more than supplementation into water after experimental infection. The plant extracts may be a good alternative to antibiotic treatment.AbstractThe study evaluated the anti-colibacteriosis efficacy of herbs in experimental infection by rabbit pathogenic strain of E. coli O103 eae+. It also studied the effects of herbal mixture added to feed or water on blood parameters. This animal model was used since some E. coli strains pathogenic for rabbits are similar to the strains that are pathogenic to humans. The components of herbal extracts were Rumex crispus, Pontentilla anserine, and Polygonum aviculare. Supplementation was carried out in water (ExpW group) or feed (ExpF group), and four weeks later the animals were infected with the E. coli O103 eae+ strain. The administration of herbs increased the mean concentration of total protein and serum albumin (p < 0.01) without causing disturbances of electrolyte and acid-base balance. The highest total antioxidant capacity (TAS) value (p < 0.01) was observed in the ExpF group. The administration of a mixture of herbs and feed caused more reduction in the number of E. coli in cecum than supplementation into water after an experimental infection. The herbs applied in rabbits did not harm the secretory functions of liver, electrolyte, and acid-base balance of the blood. The application of the tested herbal mixtures can control the activity of the intestinal microbial community.

The objective of this study was to determine the effects of feeding a protein–iron complex (PIC) to calves. Specifically, the aim was to understand how it influences productive performance and indicators of iron metabolism, hematology and biochemical and parameters during feeding with whole milk before weaning. The study was carried out on 20 Polish Holstein Friesian calves. The calves were then divided into a control group (CON), fed with full milk (n = 10), and an experimental group (MFe), who received a PIC additive in milk at 16 g/day (n = 10). In order to determine the production parameters, the calves were weighed at the beginning (i.e., on the 7th day of life) and at the end of the experiment (42nd day of life) using an electronic platform scale. Production parameters such as average weight gain (AWG), feed conversion ratio (FCR), and growth rate (GR) were assessed. Blood was collected from an external jugular vein (vena jugularis externa) on the 7th, 14th, 28th, and 42nd days of life. The mean daily gains in body weight (ADG), growth rate (GR), and the feed conversion ratio were highest in the experimental group, MFe. Therefore, it can be concluded that the addition of a protein–iron complex entailed a significant impact on the iron metabolism indicators in the MFe experimental group.