Dysfunctional uterine bleeding is a type of abnormal uterine bleeding where vaginal bleeding occurs outside of the menstrual cycle in the absence of any known pelvic pathology. Dysfunctional uterine bleeding can be treated safely with hormone therapy. Combined oral contraceptives help in increased menstrual cycle regularity and decreased blood loss. In this study, a reliable drug for the dysfunctional uterine bleeding with maximum effectiveness and minimal side effects were assessed. This study was conducted on 120 cases of dysfunctional uterine bleeding. Patients who were diagnosed with dysfunctional uterine bleeding were randomly assigned into two groups. Group D and group L included patients who were given Ethinyl estradiol 0.02mg + desogestrel 0.15mg and Ethinyl estradiol 0.03mg + levonorgestrel 0.15mg respectively for the four consecutive 28- day cycles. Menstrual blood loss was assessed using the pictorial blood assessment chart (PBAC) score on 2nd and 4th months of recruitment. Side effects such as weight gain, acne and headache were assessed in both groups. This study shows 56.68% reduction in mean PBAC score in 2 months in desogestrel group whereas only 44.96% reduction in levonorgestrel group and 79.87% reduction in mean PBAC score in desogestrel group in 4 months whereas only 74.46% reduction in levonorgestrel group. Side effects like weight gain, acne and headache were more prominent in the levonorgestrel group than desogestrel group. Desogestrel containing combined oral contraceptive can be a useful and safe treatment for dysfunctional uterine bleeding.

Lipase enzyme which catalyses the hydrolysis of fats performs a variety of chemical reactions including esterification, trans-esterification, acidolysis and aminolysis. Lipases are widely used for biotechnological application in dairy industry, oil processing and production of surfactants and preparation of enatiomerically pure pharmaceuticals. Lipases are extracted from plants, animals and microorganisms whereas high amount of lipases are produced by microbes when compared to plants and animal source. Fungi serve as a major source in the production of industrial lipase. Fungi play a vital role in removing hazardous compounds formed by crude oil contamination from oil spill during lipase extraction. Aspergillus flavus, Aspergillus niger, Penicillium restrictum, Rhizomucar rhizopodiformis, Rhizopus oligosporous, Candida rugosa, Rhizopus oryzae, Fusarium oxysporum are few fungal species involved in the production of lipase. Among the species, Aspergillus species produce efficient amount of lipase. Lipase extracted from fungi is more stable and have more diverse properties compared to lipase from other source. Fungal lipases stand out as major source because of their catalytic activity, low cost of production and relative ease in genetic manipulation. This review focuses on the various methods of lipase extraction from Aspergillus species and its applications.