No abstract

No abstract

Department o f P e d i a t r i c s , U n i v e r s i t y of Bologna. I t a l y . GH-RF d e f i c i e n t c h i l d r e n (1 u i t h i s o l a t e d GH-deficiency, 2 u i t h GH-TSH d e f i c i e n c i e s , 1 u i t h GH-TSH-ACTH-deficiencies) u e r e a d m i n i s t e r e d 5.c. hGH ( a f t e r t h e p r e v i o u s hGH t r e a t m e n t had been d i s c o n t i n u e d 1 week before the s t u d y ) a t a dose o f 0.1 UI/Kg. according t o u s u a l s u b s t i t u t i v e therapy. a t t i m e s 0 and 48 h. A f t e r a f u r t h e r week n i t h o u t t h e r a p y the s u b j e c t s u e r e a d m i n i s t e r e d i . v . GH-RF a t a dose o f 3 )g/Kg a t t i m e s 0,8.16.24,32.40.48.56,64 h . SmC b l o o d samples u e r e c o l l e c t e d a t time 0 and, a f t e r hGH and GH-RF a d m i n i s t r a t i o n s , a t t i m e s4. 8,12.16.24,28,32,36,40,48,52,56.50,64,72 h. I n a l l p a t i e n t s a s i g n i f i c a n t GH-response ( 4 n g / a l ) uas a t t a i n e d a f t e r each GH-RF a d m i n i s t r a t i o n . I n 3 s u b j e c t s a SnC i n c r e a s e was observed a f t e r both hGH and GH-RF a d m i n i s t r a t i o n s .I n 1 case t h e r e u e r e no changes i n SnC l e v e l s a f t e r both hGH and GH-RF a d m i n i s t r a t i o n s . By c a l c u l a t i n g the i n t e g r a t e d areas of the SmC curve. t h e values o b t a i n e d a f t e r hGH and GH-RF a d m i n i s t r a t i o n s u e r e h i g h e r i n 2 cases a f t e r t h e GH-RF i n j e c t i o n . h i g h e r i n 1 case a f t e r the hGH i n j e c t i o n , and i n 1 case t h e areas were s i m i l a r . I n our p a t i e n t s and i n our experimental -So far 51 children were tested in an identical mode. In the 32 cases classified as non-3 deficient all GH levels rose to more than 8 ng/ml (1. IRP) above basal with trends according to sex and age. In 19 cases GHD had been established by testing with insulln and arginine, and Sm measurements. In these patients GH increments were: <3 ng/ml in 10, 3-5 ng/ml in 6, and >9 ng/ml (14.6, 9.1, 35.8) in 3 cases. Thus, GRF(~-29) is suitable for GH testing. Easier synthesis rakes it also appear to be a favourable alternative for attempts to treat hypothalamic gravth hormone deficiency. he^ long term effect of-daily injeGions of GFS (1 ug/kg bw,sc) was studied in developing male rats.In tm additional groups GRF was stopped on day 30 and on day 50.Weight and length were m asured at 5 days intervals,GH and somtomedin =re ~asured at diverse t W pints by RIA.When cqared to the controls,GRF treated animals did not differ in length at the end of the observation pericd(day 75) .On the other hand,the growth pattern was markedly different.GRF treated animals grew faster f r m day 15 to day 25,and had significantly lower GH levels than the controls on day 30 (3.05 vs 23.0112 ng/ml,p

1H spectroscopic imaging in combination with double-quantum filtering with magnetic field gradients is used for in vivo applications in humans and animals. Because of its high selectivity and strong reduction of water signal it is also a useful tool for monitoring the distribution of specific metabolites in plants. The development and application of a double-quantum selective spectroscopic imaging sequence for detecting the sucrose distribution in the stem of sugar cane (Saccharum officinarum L.) is described. The results show that local differences in sucrose distribution can be detected non-invasively with a resolution of 0.4x0.4x6 mm(3) to 0.645x0.645x6 mm(3).

For several years, it has been possible to determine renin by a direct RIA. In the present study, plasma active renin concentration (PRC) was related to plasma renin activity (PRA) and aldosterone as a function of a standardized posture test. Using PRC, our target was to define the shortest necessary test duration. The three parameters were examined in 10 healthy male subjects (22-34 years old). Salt balance was determined in 24-hour urine, and plasma potassium and sodium were measured. Volunteers were hospitalized for 1 night, and at 8 a.m. the next morning they were subjected to the following postural changes: 3 h active orthostasis and 3 h recumbency. Frequent blood samples were taken. Orthostasis induced a significant rise in PRC, PRA and aldosterone already after 15 min. PRC and PRA reached a maximum level after 90 min of orthostasis and remained relatively stable, while aldosterone reached its highest level already after 30 min and then gradually decreased. Significant correlations were found between PRA and PRC (p < 0.001), between PRC and aldosterone (p < 0.001), and between PRA and aldosterone (p < 0.001). The PRC/PRA ratio changed during the course of the test, especially in supine subjects. When subjects returned to the supine position, all the parameters measured began a continual decrease. There were no significant changes in serum potassium and sodium levels throughout the duration of the test. It was concluded that PRC can be an easily manageable and a useful parameter in studying the renin-angiotensin-aldosterone axis and that the phase of orthostasis can be shortened to 30 min.