Direct Measurement of Immunoreactive Renin during Changes of Posture in Man

Abstract

For several years, it has been possible to determine renin by a direct RIA. In the present study, plasma active renin concentration (PRC) was related to plasma renin activity (PRA) and aldosterone as a function of a standardized posture test. Using PRC, our target was to define the shortest necessary test duration. The three parameters were examined in 10 healthy male subjects (22-34 years old). Salt balance was determined in 24-hour urine, and plasma potassium and sodium were measured. Volunteers were hospitalized for 1 night, and at 8 a.m. the next morning they were subjected to the following postural changes: 3 h active orthostasis and 3 h recumbency. Frequent blood samples were taken. Orthostasis induced a significant rise in PRC, PRA and aldosterone already after 15 min. PRC and PRA reached a maximum level after 90 min of orthostasis and remained relatively stable, while aldosterone reached its highest level already after 30 min and then gradually decreased. Significant correlations were found between PRA and PRC (p < 0.001), between PRC and aldosterone (p < 0.001), and between PRA and aldosterone (p < 0.001). The PRC/PRA ratio changed during the course of the test, especially in supine subjects. When subjects returned to the supine position, all the parameters measured began a continual decrease. There were no significant changes in serum potassium and sodium levels throughout the duration of the test. It was concluded that PRC can be an easily manageable and a useful parameter in studying the renin-angiotensin-aldosterone axis and that the phase of orthostasis can be shortened to 30 min.

Measurement of Plasma Renin Activity with Use of HPLC-Electrospray-Tandem Mass Spectrometry

“…Several methodologies for the analysis of PRA have been reported. These include RIA (1,(5)(6)(7)(8)(9)(10), HPLC-RIA (2,11,12 ), and HPLC (13)(14)(15). RIA methods are based on competitive binding principles, and the antibodies used can undergo nonspecific binding with other endogenous angiotensins.…”