Fluorescent sensor for thiols: Deprotection of nonfluorescent 1 by thiols (R′SH) proceeds rapidly and near‐quantitatively in aqueous solution (pH 7.4) to produce highly fluorescent 2. Assays performed in the presence of 1 provide a rapid and simple method for the determination of inhibitory constants for inhibitors such as donepezil toward acetyl‐ and butyrylcholinesterases.

Simply the BESThio: The fluorescein derivative BESThio (see scheme) operates as a thiol probe at pH 7.4 and functions as a selenol probe at pH 5.8, and can thus be selectively applied to the rapid quantification of selenocysteine and the determination of the selenocysteine content in selenoproteins such as glutathione peroxidase and thioredoxin reductase with high sensitivity.

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Sonde in BESThio‐Form: Das Fluorescein‐Derivat BESThio (siehe Schema) erkennt Thiolgruppen bei pH 7.4 und Selenolgruppen bei pH 5.8. Daher kann es selektiv für die schnelle Quantifizierung von Selenocystein und die hoch empfindliche Bestimmung des Selenocystein‐Gehalts in Selenoproteinen wie der Glutathion‐Peroxidase und der Thioredoxin‐Reduktase angewendet werden.

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