Aims: The objective of this work is to contribute to the valorization of medicinal and aromatic plants of the Ivorian flora. We propose to determine the chemical composition and to evaluate the antioxidant activity by spectrophotometry of the essential oil of Cardiospermum. grandiflorum Sw Study Design: Valorization of aromatic and medicinal plants. Methodology: The technical of steam distillation using a four-compartment stainless steel device was used to extract the essential oil from the plant matrix. The analysis of the essential oils was carried out on a GC chromatograph (7890A, Agilent Technologies) coupled to a mass spectrometer (5975C, Agilent Technologies). The identification of the compounds was carried out by comparison of the retention indices and mass spectra obtained with those from the National Institute of Standards and Technology (NIST) database and from the literature The antioxidant potential of the extracts was evaluated using the Blois method. Results: The essential oil obtained by steaming, with an aromatic odor and pale yellow color has a yield of (0.0045 ± 0.0002)%. Analysis of the chromatogram and mass spectra obtained by GC-MS identified 24 phytocompounds representing 99.45% of the total chemical composition. The chemical composition of EO consists mainly of hydrocarbon sesquiterpenes (97.72%).The major compound is γ-muurolene (46.06%) (A) followed by β-Caryophyllene (24.35%) (B) and γ-elemene (7.07 %). The essential oil extract of C. grandiflorum exhibits low antioxidant activity compared to vitamin C. The IC 50 value of vitamin C is 0.31 µg / mL while that of EO extract of C grandiflorum is 15.1 µg / mL Conclusion: In the present study, we are interested in the valuation of Cardiospermum grandiflorum an aromatic plant used in traditional Ivorian medicine. The yields of essential oil is low. (24) phytocompounds were identified there. The essential oil has less antioxidant activity than that of vitamin C,
In this study, we were interested in the valuation of Ocimum gratissimum, an aromatic and medicinal plant of the Ivorian flora. The essential oils of the leafy branches of Ocimum gratissimum were extracted by hydrodistillation with a Clévenger-type device. The yield of the oils obtained increases from (0.05±0.01%) to (0.42±0.08%) depending on the drying time. The density of essential oils is almost constant at 0.80. The chemical composition of essential oils was identified after analysis of chromatograms and mass spectra. The number of phytocompounds also increases with the number of drying days from 32 to 37. These compounds are marked by the presence of hydrocarbon monoterpenes from (29.11% to 49.1%), and oxygenated from (40.33% to 50.02%) and the presence of hydrocarbon sesquiterpenes from (5.52% to 8.55%), and oxygenated from (0.80% to 2.16%). The number of major compounds is 5 on day 0 and decreases to 4 on the other days, and have the following proportions: thymol from (33.60% to 44.73%), β‑caryophyllene from (2.54% to 3.29%), para-cymene (12.32% to 25.60%), γ-terpinene (10.09% to 11.41%), Bis(2-ethylhexyl)phthalate (12.12% to 0.42%). bis (2-ethylhexyl) phthalate which is in the minority during the other drying days.
Aims: The objective of this work is to contribute to the valorization of medicinal and aromatic plants of the Ivorian flora. We propose to evaluate the antioxidant and antibacterial activities of the essential oil (EO) of three species used in traditional medicine. Study Design: Valorization of aromatic and medicinal plants and essential oil. Methodology: The antioxidant potential of the extracts was evaluated using the Blois method. The antibacterial activity of the different oils at different concentrations was determined for each bacterial strain, by the technique of macro-dilution in solid medium (diffusion in wells).The minimum inhibitory concentration (MIC) and The Minimum Bactericidal Concentration (MBC) were determined. MBC / MIC ratios were calculated. When this ratio is less than 4, the extract is considered to be bactericidal. When it is 4, the extract is considered bacteriostatic. Results: The antioxidant activity has pointed out the poor antioxidant power of the essential oil(EO) extracted. The EC50 values vary from 15 µg/mL to 32 µg/mg. The antibacterial tests have shown that the samples exert an inhibitory effect on Gram (+) bacteria. The diameters of the inhibition zones vary between 14 and 25 mm for the extracts against 35 mm for the gentamycin. The MBC/MIC is 2 for the gentamycin and 4 for all the EO combined versus the resistant S. aureus methicillin. Therefore, the essential oil has shown a bacteriostatic effect on this strain. As far as S. aureus CIP 483 is concerned, the MBC/MIC has given 1 for the gentamycin, 2 for M. scandens. The EO extract of M. scandens has a bactericidal action against this bacteria strain. Conclusion: All the essential oils have less antioxidant activity than that of vitamin C. The antibacterial activity of EO has given satisfactory results on all Gram (+) bacteria. Melanthera scandens Essential Oil shows antibacterial potential against Staphylococcus aureus CIP 483.
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