This study was conducted with the objective of contributing to the valorization of aromatic and medicinal plants of Côte d’Ivoire. The essential oil (EO) of the leaves of two species of Premna, P. angolensis and P. quadrifolia obtained through steam distillation process, were analized through gas chromatography coupled with mass spectroscopy (GC-MS) and their antimicrobial activities was evaluated by agar diffusion method. The yields of EO extracted from the leaves of P. angolensis and P. quadrifolia are respectively 0.10 ± 0.02% and 0.40 ± 0.02%. 99.43% of the total chemical composition of the EO of P. angolensis leaves, are composed mainly of hydrocarbon sesquiterpenes (71.90%) followed by oxygenates (14.16%) and monoterpenes (13.22%). The main compounds found are β-caryophyllene (33.07%) and Humulene (10.78%). The EO of P. quadrifofia leaves, 99.59% of the composition was identified. It contains hydrocarbon sesquiterpenes (89.15%), followed by oxygenates compounds (8.61%) and monoterpenes (1.83%). The major compounds are αbulnesene (23.33%), germacrene D (18.83%) and caryophyllene (18.06%). The antimicrobial tests have shown that EO of P. quadrifolia leaves has no activites on studied strains and P. angolensis oil has a mild activity on Staphylococcus epidermidis and Klebsielle pneumoneae and a weak sensibility on Staphylococcus aureus CIP 4.83 and Candida albicans ATCC 10231. This study highlights chemical composition of the EO from P. angolensis and P. quadrifolia leaves. It shows more similarities of the chemical compositions of these two species of Premna and the interesting antimicrobial activity potential of P. angolensis EO
Introduction: The aromatic plants have occupied an important place in the daily life of man. The excessive and repetitive use of drugs has led the body to develop resistance to their curative effects. Many authors have focused on the study of the therapeutic properties of essential oils in order to provide new remedies. They are used as a source of bioactive molecules of natural origin. Objective: The objective of this work is to contribute to the valorization of medicinal and aromatic plants of the Ivorian flora. We propose to determine the chemical composition and to evaluate the antioxidant activity by spectrophotometry of the essential oil. Materials and Methods: The plant material consists of the leafy twigs of C. odorata. The technical of steam distillation using a four-compartment stainless steel device was used to extract the essential oil from the plant matrix. The analysis of the essential oils was carried out on a GC chromatograph (7890A, Agilent Technologies) coupled to a mass spectrometer (5975C, Agilent Technologies). The antioxidant potential of the extracts was evaluated using the Blois method. Results: The essential oil obtained by steaming, with an aromatic odor and pale green color has a yield of (0.082 ± 0.004) %. Analysis of the chromatogram and mass spectra identified 24 phytocompounds (99.92%). The phytochemical composition is dominated by hydrocarbon sesquiterpenes (44.21%) followed by hydrocarbon monoterpenes (29.48%) and other compounds (26.23%). The major compound are α-pinene (17.79%), 6-propen-1-enylbicyclo [3.1.0] and hexan-2-one (14.95%) . Conclusion: The essential oil extract of C. odorata exhibits low antioxidant activity compared to vitamin C.
L'huile essentielle de la partie aérienne de Porophyllum ruderale (Jacq.) Cass. (Asterales ; Asteraceae) obtenue par entraînement à la vapeur a été analysée par Chromatographie en Phase Gazeuse couplée à la Spectrométrie de Masse. L'activité antimicrobienne de l'huile essentielle a été évaluée sur des bactéries et champignons de référence du Centre Suisse de Recherches Scientifiques en Côte d'Ivoire. L'huile essentielle est constituée essentiellement de monoterpènes (94,69%), d'hydrocarbures aromatiques (4,39%) et de sesquiterpènes (0,27%). Les composés majoritaires sont βmyrcène (35,52%), γ-terpinène (26,37%), β-pinène (21,65%), limonène (9,26%) et 1-undécène (4,39%). L'huile essentielle a inhibé la plupart des souches testées, Klebsiella pneumoneae, Bacillus subtilus CIP, Staphylococcus auréus, Staphylococcus épidermidis, Candida albicans, Candida tropicalis et Candida glabrata, avec une concentration minimale inhibitrice (CMI) de 3,75mg/ml. Cependant , elles ne possèdent aucun effet sur Salmonella typhimirium SO 66, Eschérichia coli ATCC 25922 et Pseudomonas aéruginosa ATCC 27853. Cette étude a revélé la composition chimique de l'huile essentielle de la partie aérienne de P. ruderale. Elle a en outre montré le bon potentiel antimicrobien de l'huile essentielle.
Aims: The objective of this work is to contribute to the valorization of medicinal and aromatic plants of the Ivorian flora. We propose to determine the chemical composition and to evaluate the antioxidant activity by spectrophotometry of the essential oil of Cardiospermum. grandiflorum Sw Study Design: Valorization of aromatic and medicinal plants. Methodology: The technical of steam distillation using a four-compartment stainless steel device was used to extract the essential oil from the plant matrix. The analysis of the essential oils was carried out on a GC chromatograph (7890A, Agilent Technologies) coupled to a mass spectrometer (5975C, Agilent Technologies). The identification of the compounds was carried out by comparison of the retention indices and mass spectra obtained with those from the National Institute of Standards and Technology (NIST) database and from the literature The antioxidant potential of the extracts was evaluated using the Blois method. Results: The essential oil obtained by steaming, with an aromatic odor and pale yellow color has a yield of (0.0045 ± 0.0002)%. Analysis of the chromatogram and mass spectra obtained by GC-MS identified 24 phytocompounds representing 99.45% of the total chemical composition. The chemical composition of EO consists mainly of hydrocarbon sesquiterpenes (97.72%).The major compound is γ-muurolene (46.06%) (A) followed by β-Caryophyllene (24.35%) (B) and γ-elemene (7.07 %). The essential oil extract of C. grandiflorum exhibits low antioxidant activity compared to vitamin C. The IC 50 value of vitamin C is 0.31 µg / mL while that of EO extract of C grandiflorum is 15.1 µg / mL Conclusion: In the present study, we are interested in the valuation of Cardiospermum grandiflorum an aromatic plant used in traditional Ivorian medicine. The yields of essential oil is low. (24) phytocompounds were identified there. The essential oil has less antioxidant activity than that of vitamin C,
In this study, we were interested in the valuation of Ocimum gratissimum, an aromatic and medicinal plant of the Ivorian flora. The essential oils of the leafy branches of Ocimum gratissimum were extracted by hydrodistillation with a Clévenger-type device. The yield of the oils obtained increases from (0.05±0.01%) to (0.42±0.08%) depending on the drying time. The density of essential oils is almost constant at 0.80. The chemical composition of essential oils was identified after analysis of chromatograms and mass spectra. The number of phytocompounds also increases with the number of drying days from 32 to 37. These compounds are marked by the presence of hydrocarbon monoterpenes from (29.11% to 49.1%), and oxygenated from (40.33% to 50.02%) and the presence of hydrocarbon sesquiterpenes from (5.52% to 8.55%), and oxygenated from (0.80% to 2.16%). The number of major compounds is 5 on day 0 and decreases to 4 on the other days, and have the following proportions: thymol from (33.60% to 44.73%), β‑caryophyllene from (2.54% to 3.29%), para-cymene (12.32% to 25.60%), γ-terpinene (10.09% to 11.41%), Bis(2-ethylhexyl)phthalate (12.12% to 0.42%). bis (2-ethylhexyl) phthalate which is in the minority during the other drying days.
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