Exposure of the developing organism to industrial chemicals and physical factors represents a serious risk factor for the development of neurobehavioral disorders, such as attention-deficit hyperactivity disorder, autism and mental retardation. Appropriate animal models are needed to test potentially harmful effects and mechanisms of developmental neurotoxicity of various chemical substances. However, there are significant human vs. rat differences in the brain developmental profile which should be taken into account in neurotoxicity studies. Subtle behavioral alterations are hard to detect by traditional developmental toxicity and teratogenicity studies, and in many cases they remain hidden. They can however be revealed by using special behavioral, endocrine and/or pharmacological challenges, such as repeated behavioral testing, exposure to single stressful stimulus or drugs. Further, current neurobehavioral test protocols recommend to test animals up to their adulthood. However some behavioral alterations, such as anxiety-like behavior or mental deficiency, may become manifest in later periods of development. Our experimental and scientific experiences are highly suggestive for a complex approach in testing potential developmental neurotoxicity. Strong emphasis should be given on repeated behavioral testing of animals up to senescence and on using proper pharmacological and/or stressful challenges.

Stobadine (STO) is a prospective neuro‐ and cardioprotective drug with high antioxidative properties. The aim of this study was to ascertain the effect of long‐term administration of STO on exploratory behaviour and habituation processes in adult virgin female and male rats. Stobadine was administered by oral gavage in a single dose of 50 mg kg−1 day−1 for a total of 56 days. The animals were tested for exploratory behaviour—intensity of motor and vertical activity in an open field test in three blocks of measurements (initial screening; after 56 days of STO administration; and 28 days after the last treatment). The rate of decline of motor activity was evaluated during four consecutive days of testing (interrupted habituation). Administration of STO resulted in transient inhibition of exploratory behaviour in female rats without overtly detectable toxicity. Exploratory behaviour of males was not affected by STO treatment. Copyright © 1999 John Wiley & Sons, Ltd.

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