Mushroom cultivation on a cost-effective and easily accessible substrate is one of the key areas of research of mycologists. In this present investigation, agro-industrial wastes have been used as a substratum to produce protein-enriched mushrooms having different medicinal potentiality with respect to growth, productivity, and biological efficiency. Mycelium running rate, development of fruiting bodies and productivity of Pleurotus florida was evaluated. The total running of mycelium in days, maximum primordial formation in three flushes, and total numbers of fruit bodies in three flushes were noted. In the grass substratum, fruiting body size was bigger as well as exhibited higher productivity (18.42 %) and biological efficiency (97.58%) in all three flushes and the least productivity and biological efficiency observed in sugarcane bagasse. The current study reveals that the various types of substrates affect mushroom growth, productivity, and biological efficiency.

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